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> Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His)

Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His)

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Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His)

(Synonyms: interferon, α1, interferon, alpha 1, Interferon α1) Copy Product Info

Synonyms: interferon, α1, interferon, alpha 1, Interferon α1

Catalog No. TMPY-02865 Copy Product Info
Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.9 kDa and the accession number is P05011.
Other Forms of Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His):
Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (hFc)
Interferon alpha 1/IFNA1 Protein, Human, Recombinant (His)
Interferon alpha 1/IFNA1 Protein, Mouse, Recombinant (His)
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 4-20 ng/mL.
Description
Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.9 kDa and the accession number is P05011.
Species
Rat
Expression System
HEK293 Cells
TagC-His
Accession NumberP05011
ConstructionA DNA sequence encoding the rat IFNA1 (P05011) (Met1-Ser192) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Cys 24
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonymsinterferon, α1, interferon, alpha 1, Interferon α1
Research Background
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes, and they also increase the ability of uninfected host cells to resist new infection by the virus. Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has antiviral activities.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Chemical Properties
Molecular Weight20.9 kDa (predicted); 20-25 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

interferon, α 1interferon, a 1
Related Tags: Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His) chemical structure | Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (His) molecular weight