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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human IgM heavy chain.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs,RAB,MAB,Recombinant Mab,MAB,monoclonal Ab,monoclonal antibody

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against human IgM.

    Reactivity

    Human

    Specificity

    This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (50-200 ng/well (for Capture); 0.05-0.2 ug/mL (for Detection))
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
    Immunocytochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.