Cytokine array for detection of 62 human adipokines. Suitable for all liquid sample types.
Human Obesity Array C1
Human Obesity Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 62 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Cardiovascular Disease, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, Angiopoietin-1, Angiopoietin-2, ANGPTL4, CRP (C-Reactive Protein), ENA-78 (CXCL5), Fas (TNFRSF6/Apo-1), FGF-6, Growth Hormone, HCC-4 (CCL16), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p70, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-6, IL-6 R, IL-8 (CXCL8), Insulin, IP-10 (CXCL10), Leptin R, Leptin, LIF, Lymphotactin (XCL1), MCP-1 (CCL2), MCP-3 (MARC/CCL7), M-CSF, MIF, MIP-1 beta (CCL4), MSP alpha/beta, Osteoprotegerin (TNFRSF11B), Oncostatin M, PAI-1, PARC (CCL18), PDGF-AA, PDGF-AB, PDGF-BB, RANTES (CCL5), Resistin, SAA (Serum Amyloid A), SDF-1 alpha (CXCL12 alpha), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TECK (CCL25), TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Obesity Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Obesity and p16INK4A Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity
- Species:
- Human
- Sample Type:
- Obadi cell, Leadi cell
- Year:
- 2023
- Journal Name:
- Molecular and Cellular Biology
Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
A nutritional nonalcoholic steatohepatitis minipig model
- Species:
- Porcine
- Sample Type:
- Plasma (Minipig dietary model)
- Year:
- 2016
- Journal Name:
- Journal of Nutritional Biochemistry
- PubMed ID:
- 26878782
miR-34a inhibits differentiation of human adipose tissue-derived stem cells by regulating cell cycle and senescence induction
- Species:
- Human
- Sample Type:
- Conditioned Media (Adipose tissue lysate stem cells)
- Year:
- 2016
- Journal Name:
- Differentiation
- PubMed ID:
- 26677981
Diallyl Disulfide Inhibits TNF-induced CCL2 Release by MDA-MB-231 Cells
- Species:
- Human
- Sample Type:
- Conditioned Media (MDA-MB-231 Cells)
- Year:
- 2014
Characterization of a novel proinflammatory effect mediated by BK and the kinin B2 receptor in human preadipocytes.
- Species:
- Human
- Sample Type:
- Conditioned Media (pre-adipocytes obesity model)
- Year:
- 2013
- Journal Name:
- Biochemical Pharmacology
- PubMed ID:
- 23796753
Breast stromal fibroblasts from histologically normal surgical margins are procarcinogenic.
- Species:
- Human
- Sample Type:
- Cell Lysate (SFCMs from CAF, TCF and NBF cells)
- Year:
- 2013
- PubMed ID:
- 24009142
