RayBio® Human MCP-2 (CCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human MCP-2 ELISA Kit
Human MCP-2 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1.5 pg/ml - 200 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 - 5 fold |
| Accession Number | P80075 |
| Gene Id | 6355 |
| Gene Symbols | CCL8, MCP2, SCYA10, SCYA8 |
| Protein Name / Synonyms | C-C motif chemokine 8 (HC14) (Monocyte chemoattractant protein 2) (Monocyte chemotactic protein 2) (MCP-2) (Small-inducible cytokine A8) [Cleaved into: MCP-2(6-76)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human MCP-2 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MCP-2 (CCL8), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
A Glucuronic Acid-Palmitoylethanolamide Conjugate (GLUPEA) Is an Innovative Drug Delivery System and a Potential Bioregulator
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell supernatants from HaCaT keratinocytes)
- Year:
- 2021
- Journal Name:
- Cells
- PubMed ID:
- 33672574
CaSR Antagonist (Calcilytic) NPS 2143 Hinders the Release of Neuroinflammatory IL-6, Soluble ICAM-1, RANTES, and MCP-2 from Aβ-Exposed Human Cortical Astrocytes
- Species:
- Human
- Sample Type:
- Other / Unspecified (NAHAs-conditioned growth media)
- Year:
- 2020
- Journal Name:
- Cells
- PubMed ID:
- 32498476
Chemokine levels in serum of children with atopic dermatitis with regard to severity and sensitization status
- Species:
- Human
- Sample Type:
- Serum (Atopic Dermatitis and chemockine levels)
- Year:
- 2015
- PubMed ID:
- 26102348
Large-scale gene analysis of rabbit atherosclerosis to discover new biomarkers for coronary artery disease
- Species:
- Human
- Sample Type:
- Serum (coronary artery disease patients)
- Year:
- 2019
- Journal Name:
- Open Biology
- PubMed ID:
- 30958112
Low serum levels of myeloid progenitor inhibitory factor-1 predict good response to methotrexate in rheumatoid arthritis.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- International Scholarly Research Notices Inflammation
- PubMed ID:
- 24455421
Latency-associated viral IL-10 encoded by human cytomegalovirus modulates cellular IL-10 and CCL8 secretion during latent infection through changes in the cellular miRNA, has miR-92a
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2014
- Journal Name:
- Journal of Virology
Phosphatase inhibitor, sodium stibogluconate, in combination with interferon (IFN) alpha 2b: phase I trials to identify pharmacodynamic and clinical effects.
- Species:
- Human
- Sample Type:
- Serum (patients with metastatic melanoma, soft tissue sarcomas, gastrointestinal stromal tumors, and breast or colorectal carcinomas)
- Year:
- 2011
- Journal Name:
- Oncotarget
Bone Morphogenetic Protein Signaling in Vascular Disease: ANTI-INFLAMMATORY ACTION THROUGH MYOCARDIN-RELATED TRANSCRIPTION FACTOR A
- Species:
- Human
- Sample Type:
- Conditioned Media (Pulmonary artery smooth muscles cell cultures)
- Year:
- 2012
- Journal Name:
- The Journal of Biological Chemistry
Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins
- Species:
- Human
- Sample Type:
- Conditioned Media (U937 Cells)
- Year:
- 2012
- Journal Name:
- Biochemical Journal
- PubMed ID:
- 22060158
Adelmidrol increases the endogenous concentrations of palmitoylethanolamide in canine keratinocytes and down-regulates an inflammatory reaction in an in vitro model of contact allergic dermatitis
- Species:
- Human
- Sample Type:
- Conditioned Media (Poly IC stimulated HaCaT cells with or without PEA, human keratinocytes)
- Year:
- 2016
- Journal Name:
- Veterinary Journal
- PubMed ID:
- 26639824
