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> Human IL-8 ELISA Kit

Human IL-8 ELISA Kit

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Referencia ELH-IL8-5

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Marca : RayBiotech

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Human IL-8 ELISA Kit Standard CurveHuman IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA KitELH-IL8 was used to measure IL-8 in plasma and serum samples from patients with non-small cell lung cancer compared to normal individuals. Figure adapted from Lim et al., Diseases, 2024.ELH-IL8 was used to measure CXCL8 in conditioned media from tumour cell lines and extracellular vesicle–treated lymphatic endothelial cells. Figure adapted from Su et al., Journal of Extracellular Vesicles, 2023.ELH-IL8 was used to measure CXCL8 in colon cancer cell culture supernatants under SKAP1 overexpression or knockdown conditions. Figure adapted from Gao et al., Adv Sci (Weinh)., 2024.ELH-IL8 was used to measure IL-8 in culture supernatants of LPS-stimulated Caco-2 cells treated with bioaccessible fractions of pomegranate seed oils. Figure adapted from Banares et al., Food Research International, 2023.IL-8 levels in trophoblast cell culture supernatants were quantified using a RayBiotech Human IL-8 ELISA Kit to evaluate the effect of miR-95-3p and EPM2A modulation in severe preeclampsia. Figure adapted from Gao et al., Arch Biochem Biophys, 2023.ELH-IL8 was used to measure IL-8 in conditioned media from human mesenchymal stem cells, degenerative disc cells, and 1:1 co-cultures treated with o-Vanillin. Figure adapted from Li et al., Cells, 2022.Human IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA Kit5 Plate Kit Packaging (one box)

Human IL-8 ELISA Kit Standard Curve

Human IL-8 ELISA Kit

RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
SpeciesHuman
Sensitivity
1 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
1 pg/ml - 600 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P10145
Gene Id
3576
Gene Symbols
CXCL8, IL8
Protein Name / Synonyms
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Cancer, Inflammation, Antibody Drug Development, Protein Therapeutics, Chemokine Biology
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Interleukin 8 (IL-8) is a chemotactic factor that attracts neutrophils, basophils and T-cells during an inflammatory process. It is released from a variety cell types such as monocytes, macrophages, neutrophils and intestine, kidney, placenta and bone marrow cells in response to inflammation. It is involved many cellular processes including cell proliferation, tissue remodeling and angiogenesis. IL-8 mediates its effects by binding to two heterotrimeric G protein-coupled receptors, CXCR1 and CXCR2. Given that it is a major mediator of inflammation, IL-8 was markedly increased in infants diagnosed with perinatal infection.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-IL8 was used to measure IL-8 in plasma and serum samples from patients with non-small cell lung cancer compared to normal individuals. Figure adapted from Lim et al., Diseases, 2024.

ELH-IL8 was used to measure CXCL8 in conditioned media from tumour cell lines and extracellular vesicle–treated lymphatic endothelial cells. Figure adapted from Su et al., Journal of Extracellular Vesicles, 2023.

ELH-IL8 was used to measure CXCL8 in colon cancer cell culture supernatants under SKAP1 overexpression or knockdown conditions. Figure adapted from Gao et al., Adv Sci (Weinh)., 2024.

ELH-IL8 was used to measure IL-8 in culture supernatants of LPS-stimulated Caco-2 cells treated with bioaccessible fractions of pomegranate seed oils. Figure adapted from Banares et al., Food Research International, 2023.

IL-8 levels in trophoblast cell culture supernatants were quantified using a RayBiotech Human IL-8 ELISA Kit to evaluate the effect of miR-95-3p and EPM2A modulation in severe preeclampsia. Figure adapted from Gao et al., Arch BiochemBiophys, 2023.

ELH-IL8 was used to measure IL-8 in conditioned media from human mesenchymal stem cells, degenerative disc cells, and 1:1 co-cultures treated with o-Vanillin. Figure adapted from Li et al., Cells, 2022.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human IL-8 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IL-8 (IL8 / CXCL8), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

citations

  • Perivascular cells function as key mediators of mechanical and structural changes in vascular capillaries

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (supernatants of biological replicate chips)
    Year:
    2025
    Journal Name:
    Science Advances
    PubMed ID:
    39792671

  • GPRC5A/CXCL8/NLRP3-mediated neutrophil extracellular traps drive gemcitabine-nab-paclitaxel resistance in pancreatic adenocarcinoma

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants of GnP-resistant MIA PaCa-2 or PANC-1 cells)
    Year:
    2025
    Journal Name:
    Cancer Biology & Medicine
    PubMed ID:
    40665634

  • Targeting polymicrobial skin infections in atopic dermatitis: role of Pichia kudriavzevii postbiotics as innovative tools for human health

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants HaCaT)
    Year:
    2026
    Journal Name:
    Frontiers in Microbiology
    PubMed ID:
    42625856

  • ASC-Derived Extracellular Vesicles Suppress Macrophage-Driven Inflammatory Amplification and Contractile Activation of Uterine Smooth Muscle Cells

    Year:
    2026
    Journal Name:
    International Journal of Molecular Sciences
    PubMed ID:
    42196254

  • Adjunctive effect of gaseous ozone on inflammatory biomarkers and microbial profile in grade C molar-incisor pattern periodontitis: a randomized split-mouth placebo-controlled clinical trial

    Species:
    Human
    Sample Type:
    Other Body Fluid (sample strips eluate gingival crevicular fluid)
    Year:
    2026
    Journal Name:
    BMC oral health
    PubMed ID:
    41942979

  • Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (conditioned media from breast cancer cell lines)
    Year:
    2026
    Journal Name:
    International Journal of Molecular Sciences
    PubMed ID:
    41596270

  • Interleukin-8 Expression Under Highly Effective Host Immunological Control of HIV-1 Disease Progression

    Species:
    Human
    Sample Type:
    Serum (HIV-1 infected individuals)
    Year:
    2026
    Journal Name:
    Iconic Research and Engineering Journals

  • Stacked human aortic endothelial cells induce atherosclerotic fatty streaks and release proinflammatory cytokines and chemokines

    Year:
    2026
    Journal Name:
    Mechanobiol Med
    PubMed ID:
    42088731

  • Tumour extracellular vesicles induce neutrophil extracellular traps to promote lymph node metastasis

    Species:
    Human
    Sample Type:
    BEAS-2B cell, A549 cell
    Year:
    2023
    Journal Name:
    Journal of Extracellular Vesicles

  • Mechanism of PAVA-induced toxicity and inflammation in a cocultured skin cell model

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (co-cultured keratinocyte, dermal fibroblast culture supernatants)
    Year:
    2025
    Journal Name:
    Frontiers in Pharmacology
    PubMed ID:
    40041491

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