RayBio® Human IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human IL-17 ELISA Kit
Human IL-17 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 80 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 80 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q16552 |
| Gene Id | 3605 |
| Gene Symbols | IL17A, IL17, CTLA8 |
| Protein Name / Synonyms | Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human IL-17 qIPCR ELISA Kit
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IL-17 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-17A (IL17A), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Serum NEAT1/MEG3 and miR-124/miR-146a Dysregulation in Behçet's Disease: Association with Th17/NF-κB-Related Inflammation and Diagnostic Potential
- Species:
- Human
- Sample Type:
- Serum (Behçet's disease patients vs controls)
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 42589375
RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma
- Species:
- Human
- Sample Type:
- oral squamous cell carcinoma (OSCC) tissue
- Year:
- 2022
- Journal Name:
- Cells
Metformin may induce immunologic tolerance at the maternal-foetal interface in early human pregnancy
- Species:
- Human
- Sample Type:
- decidual immune cells (DICs)
- Year:
- 2023
- Journal Name:
- American journal of translational research
Association Between IL-23 and Coronary Arterial Lesions in Children with Kawasaki Disease
- Species:
- Human
- Sample Type:
- Serum (children with kawasaki disease)
- Year:
- 2021
- Journal Name:
- Research Square
TaaI/Cdx-2 AA Variant of VDR Defines the Response to Phototherapy amongst Patients with Psoriasis
- Species:
- Human
- Sample Type:
- Whole Blood (psoriasis patients, pre/post phototherapy)
- Year:
- 2021
- Journal Name:
- Life (Basel, Switzerland)
- PubMed ID:
- 34208603
The Effect of Cannabis on the Clinical and Cytokine Profiles in Patients with Multiple Sclerosis
- Species:
- Human
- Sample Type:
- Serum (MS patients, cannabis users vs non-users)
- Year:
- 2021
- Journal Name:
- Multiple sclerosis international
- PubMed ID:
- 33628507
Linkage between IL-23 and coronary arterial lesions in pediatric patients with Kawasaki disease
- Species:
- Human
- Sample Type:
- Serum (kawasaki disease, febrile and healthy controls)
- Year:
- 2026
- Journal Name:
- Frontiers in Pediatrics
- PubMed ID:
- 41809521
mRNA expression analysis of interleukins 17A and 17F in bronchial asthmatic patients from Northern Indian population
- Species:
- Human
- Sample Type:
- Serum (asthma patients and controls)
- Year:
- 2020
- Journal Name:
- Journal of family medicine and primary care
- PubMed ID:
- 32754484
Fat mass and obesity-associated protein regulates tumorigenesis of arecoline-promoted human oral carcinoma
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (tumor tissues)
- Year:
- 2021
- Journal Name:
- Cancer Medicine
- PubMed ID:
- 34378866
Allergic Rhinitis and Its Relationship with IL-10, IL-17, TGF-, IFN-, IL 22, and IL-35
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2018
- Journal Name:
- Disease Markers
- PubMed ID:
- 29692871
