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Human IL-13 ELISA Kit
Human IL-13 ELISA Kit
RayBio® Human IL-13 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.15 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.15 pg/ml - 40 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P35225 |
| Gene Id | 3596 |
| Gene Symbols | IL13, NC30 |
| Protein Name / Synonyms | Interleukin-13 (IL-13) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human IL-13 qIPCR ELISA Kit
As low as $639.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IL-13 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-13 (IL13), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Role of ILC2s as Potential Effector Cells of IL25-Mediated Type 2 Inflammation in Chronic Rhinosinusitis with Nasal Polyps in China
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants of extraction of nasal polyps, ILC2s cell-culture supernatants)
- Year:
- 2025
- Journal Name:
- Journal of Inflammation Research
- PubMed ID:
- 40823358
Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (SSc patient PBMCs treated with IL-40)
- Year:
- 2025
- Journal Name:
- Arthritis Research & Therapy
- PubMed ID:
- 40457473
Inhibition of human macrophage activation via pregnane neurosteroid interactions with toll-like receptors: Sex differences and structural requirements
- Species:
- Human
- Sample Type:
- human monocyte-derived macrophages (hMDM)
- Year:
- 2022
- Journal Name:
- Frontiers in immunology
A Proteomics Investigation of Cigarette Smoke Exposed Wistar Rats Revealed Improved Anti-Inflammatory Effects of the Cysteamine Nanoemulsions Delivered via Inhalation
- Species:
- Human
- Sample Type:
- BEAS2B cell
- Year:
- 2023
- Journal Name:
- Omics-a Journal of Integrative Biology
Clinical and Biological Markers of Frailty Syndrome in Patients Undergoing Elective Percutaneous Coronary Intervention
- Species:
- Human
- Sample Type:
- Serum (CAD patients before PCI)
- Year:
- 2024
- Journal Name:
- Diagnostics
- PubMed ID:
- 39682572
Myeloid-Derived Suppressor Cells Promote the Progression of Primary Membranous Nephropathy by Enhancing Th17 Response
- Species:
- Human
- Sample Type:
- Plasma (primary membranous nephropathy patients)
- Year:
- 2020
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 32973748
Mesenchymal stem cells regulate type 2 innate lymphoid cells via regulatory T cells through ICOS-ICOSL interaction
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatant of PBMCs)
- Year:
- 2021
- Journal Name:
- Stem Cells
- PubMed ID:
- 33662168
Beclomethasone dipropionate and sodium cromoglycate protect against airway hyperresponsiveness in a human ex vivo model of cow's milk aspiration
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants of human isolated bronchi)
- Year:
- 2021
- Journal Name:
- Current research in pharmacology and drug discovery
- PubMed ID:
- 34909646
Decreased Levels of Microfibril-Associated Glycoprotein (MAGP)-1 in Patients with Colon Cancer and Obesity Are Associated with Changes in Extracellular Matrix Remodelling
- Species:
- Human
- Sample Type:
- Whole Blood (circulating blood)
- Year:
- 2021
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 34445187
Role of Serum Vitamin D, Interleukin 13, and microRNA-135a in Hepatocellular Carcinoma and Treatment Failure in Egyptian HCV-Infected Patients Receiving Direct Antiviral Agents
- Species:
- Human
- Sample Type:
- Serum (HCV patients on DAAs)
- Year:
- 2021
- Journal Name:
- Viruses
- PubMed ID:
- 34696438
