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Human IGFBP-5 ELISA Kit
Human IGFBP-5 ELISA Kit
RayBio® Human IGFBP-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 ng/ml - 800 ng/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P24593 |
| Gene Id | 3488 |
| Gene Symbols | IGFBP5, IBP5 |
| Protein Name / Synonyms | Insulin-like growth factor-binding protein 5 (IBP-5) (IGF-binding protein 5) (IGFBP-5) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | IGF Signaling |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human IGFBP-5 qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IGFBP-5 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IGFBP-5 (IGFBP5), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone
- Year:
- 2024
- Journal Name:
- eBioMedicine
- PubMed ID:
- 38772281
Targeting Mybbp1a suppresses HCC progression via inhibiting IGF1/AKT pathway by CpG islands hypo-methylation dependent promotion of IGFBP5
- Species:
- Human
- Year:
- 2019
- Journal Name:
- eBioMedicine
- PubMed ID:
- 31109829
Proteomics discovery of pulmonary hypertension biomarkers: Insulin-like growth factor binding proteins are associated with disease severity
- Species:
- Human
- Year:
- 2022
Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (PBS release buffer from ECM-based TEBs)
- Year:
- 2020
- Journal Name:
- Journal of tissue engineering
- PubMed ID:
- 32551034
Serum Concentrations of Insulin-like Growth Factor-binding Protein 5 in Crohn's Disease.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
Serum concentrations of human insulin-like growth factor-1 and levels of insulin-like growth factor-binding protein-5 in patients with nonalcoholic fatty liver disease: association with liver histology
- Species:
- Human
- Sample Type:
- Serum (Non alchololic Fatty Liver disease patients)
- Year:
- 2012
- Journal Name:
- European Journal of Gastroenterology and Hepatology
- PubMed ID:
- 22157234