Product Info Summary
| SKU: | EK0376 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IGF-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0376
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
IGF signaling (putative; PI3K/AKT, MAPK); roles in growth and metabolic regulation (putative). Clinical use (putative): assess growth disorders/anabolic status; kit measures free IGF-1 per on-page Q&A. Assay context: sandwich ELISA for human serum/plasma (heparin/EDTA) and supernatants; sensitivity ~50 pg/mL; range 156–10,000 pg/mL; no detectable cross-reactivity with IGF-2 (from product page). May be analyzed with IL-6 to explore immune–metabolic crosstalk (putative).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGF-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0376)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G49-A118
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0376 for more details
Cross-reactivity
There is no detectable cross-reactivity with IGF-2.
Reactive Species
EK0376 is reactive to IGF1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0376 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF1
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0376-CAP | Anti-Human IGF1 Pre-coated 96-well strip microplate | 1 |
| EK0376-ST | Human IGF1 Standard | 2 vials, 10 ng/tube |
| EK0376-DA | Human IGF1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Question
Would it would work with their bear samples?
Verified customer
Asked: 2020-09-25
Answer
The animal species trying to measure IGF1 concentrations is the Asiatic black bear (Ursus thibetanus Japonicus), and the exact protein sequencing has not been reported. In the case of closely related species, brown and polar bears have genomic information available to the public.
Boster Scientific Support
Answered: 2020-10-25