RayBio® Human IGF-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Human IGF-1 ELISA Kit
Human IGF-1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 100 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.1 ng/ml - 30 ng/ml |
| Specificity | This ELISA antibody pair only can detect a free form of human IGF-1 (not a complex such as binding with IGFBPs). This IGF-1 ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 - 20 fold |
| Accession Number | P05019 |
| Gene Id | 3479 |
| Gene Symbols | IGF1, IBP1 |
| Protein Name / Synonyms | Insulin-like growth factor I (IGF-I) (Mechano growth factor) (MGF) (Somatomedin-C) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, IGF Signaling |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human IGF-1 qIPCR ELISA Kit
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RayPlex® Human IGF-1 Bead Assay
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Target Background
Insulin-like-growth factor is a hormone structurally similar to insulin. It is central to the somatotropic axis acting downstream of growth hormone (GH) to promote tissue growth and development. IGF1 is synthesized in the liver, where its secretion is regulated by the pituitary secretion of GH. The actions of IGF1 are mediated through the IGF1R receptor to activate downstream signaling cascades such as PI3-kinase and mitogen-activated protein kinase pathways. Defects in the encoding gene can cause insulin-like growth factor deficiency - characterized by growth retardation, sensorineural deafness and intellectual disability.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IGF-1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IGF-1 (IGF1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Lifelong football training influences miR-1303 serum expression and human breast cancer MCF-7 cells motility: a pilot study
- Species:
- Human
- Sample Type:
- Serum (lifelong football players vs controls)
- Year:
- 2026
- Journal Name:
- Frontiers in Sports and Active Living
- PubMed ID:
- 42523747
Inflammatory biomarkers and sarcopenia in older adults
- Year:
- 2026
- Journal Name:
- Frontiers in Aging
- PubMed ID:
- 42267078
Establishing Predictors of Acute Sarcopenia: A Proof-Of-Concept Study Utilising Network Analysis
- Year:
- 2024
- Journal Name:
- Aging and Disease
- PubMed ID:
- 39012665
Evaluating Pro- and Anti-inflammatory Biomarkers for Predicting Type 2 Diabetes Mellitus in the Geriatric Population and Correlation With Clinical and Biochemical Parameters
- Species:
- Human
- Sample Type:
- Serum (geriatric subjects, fasting)
- Year:
- 2025
- Journal Name:
- Curēus
- PubMed ID:
- 39991369
Hydrogel-Mediated Local Delivery of Induced Nephron Progenitor Cell-Sourced Molecules as a Cell-Free Approach for Acute Kidney Injury
- Species:
- Human
- Sample Type:
- Other / Unspecified (medium conditioned by iNPCs)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 39408943
Correlation of bioactive components of platelet rich plasma derived from human female adult peripheral blood and umbilical cord blood with age
- Species:
- Human
- Sample Type:
- Platelet-rich plasma (PRP)
- Year:
- 2023
- Journal Name:
- Scientific reports
Conditioned Media from Head and Neck Cancer Cell Lines and Serum Samples from Head and Neck Cancer Patients Drive Catabolic Pathways in Cultured Muscle Cells
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Cancers
Effects of taxifolin from enzymatic hydrolysis of Rhododendron mucrotulatum on hair growth promotion
- Species:
- Human
- Sample Type:
- Human follicle dermal papilla cells (HFDPC)
- Year:
- 2022
- Journal Name:
- Frontiers in bioengineering and biotechnology
Functional Characterization of Renal Tubular Epithelial Cells in Optimized Coculture Systems
- Species:
- Human
- Sample Type:
- proximal tubule epithelial cells (RPTECs), bone marrow mesenchymal stem cells (BMMSCs)
- Year:
- 2023
- Journal Name:
- Nephron
Metal‐HisTag coordination for remote loading of very small quantities of biomacromolecules into PLGA microspheres
- Species:
- Human
- Sample Type:
- Other / Unspecified (protein released from PLGA microspheres)
- Year:
- 2021
- Journal Name:
- Bioengineering & Translational Medicine
- PubMed ID:
- 35600641
