RayBio® Human HGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human HGF ELISA Kit
Human HGF ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 3 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P14210 |
| Gene Id | 3082 |
| Gene Symbols | HGF, HPTA |
| Protein Name / Synonyms | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Autophagy, Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Human HGF qIPCR ELISA Kit
As low as $639.00
RayPlex® Human HGF Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human HGF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified HGF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
PlexinD1 is a driver and a therapeutic target in advanced prostate cancer
- Species:
- Human
- Year:
- 2025
- Journal Name:
- EMBO Molecular Medicine
- PubMed ID:
- 39748059
Hypoxia-induced histone methylation and NF-κB activation in pancreas cancer fibroblasts promote EMT-supportive growth factor secretion. bioRxiv [Preprint]
- Year:
- 2025
- Journal Name:
- bioRxiv
- PubMed ID:
- 39974981
Effect of controlled release of HGF on extracellular vesicle secretion by urine-derived stem cells
- Species:
- Human
- Sample Type:
- Other / Unspecified (samples from the soaking solution, dissolved alginate beads)
- Year:
- 2024
- Journal Name:
- Frontiers in Bioengineering and Biotechnology
- PubMed ID:
- 39234273
Xeroderma Pigmentosum Type C Primary Skin Fibroblasts Overexpress HGF and Promote Squamous Cell Carcinoma Invasion in the Absence of Genotoxic Stress
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (XP-C primary skin fibroblasts)
- Year:
- 2024
- Journal Name:
- Cancers
- PubMed ID:
- 39409898
Cholinergic signaling via muscarinic M1 receptor confers resistance to docetaxel in prostate cancer
- Species:
- Human
- Year:
- 2024
- Journal Name:
- Cell reports. Medicine
- PubMed ID:
- 38262412
Hydrogel-Mediated Local Delivery of Induced Nephron Progenitor Cell-Sourced Molecules as a Cell-Free Approach for Acute Kidney Injury
- Species:
- Human
- Sample Type:
- Other / Unspecified (medium conditioned by iNPCs)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 39408943
Modulation of Fibroblast Phenotype by Colorectal Cancer Cell-Secreted Factors Is Mostly Independent of Oncogenic KRAS
- Species:
- Human
- Sample Type:
- HCT116 cell, LS174T cell
- Year:
- 2022
- Journal Name:
- Cells
MSC-NTF (NurOwn®) exosomes: a novel therapeutic modality in the mouse LPS-induced ARDS model
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (sEV lysates)
- Year:
- 2021
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 33468250
Tumor specific cytokine pattern explored in renal cell carcinoma
- Species:
- Human
- Sample Type:
- Serum; Tissue Lysate / Homogenate; Urine
- Year:
- 2026
- Journal Name:
- PLoS ONE
- PubMed ID:
- 42809553
An optimized procedure for preparation of conditioned medium from Wharton’s jelly mesenchymal stromal cells isolated from umbilical cord
- Species:
- Human
- Sample Type:
- mesenchymal stromal cells (MSCs)
- Year:
- 2023
- Journal Name:
- Frontiers in molecular biosciences
