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Human G-CSF ELISA Kit
Human G-CSF ELISA Kit
RayBio® Human GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 500 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09919 |
| Gene Id | 1440 |
| Gene Symbols | CSF3, C17ORF33, GCSF |
| Protein Name / Synonyms | Granulocyte colony-stimulating factor (G-CSF) (Pluripoietin) (Filgrastim) (Lenograstim) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human G-CSF qIPCR ELISA Kit
As low as $639.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human G-CSF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified GCSF (CSF3), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
HPV16-Expressing Tumors Release Multiple IL1 Ligands to Orchestrate Systemic Immunosuppression Whose Disruption Enables Efficacy of a Therapeutic Vaccine
- Species:
- Human
- Sample Type:
- Serum (cervical cancer patients vs normal donors)
- Year:
- 2025
- Journal Name:
- Cancer Discovery
- PubMed ID:
- 40287949
Circulating angiogenesis-related biomarkers in sickle cell retinopathy and maculopathy
- Species:
- Human
- Sample Type:
- Plasma (sickle cell disease patients)
- Year:
- 2026
- Journal Name:
- British Journal of Haematology
- PubMed ID:
- 41715990
Increasing Age Affected Polymorphonuclear Neutrophils in Prognosis of Mycoplasma pneumoniae Pneumonia
- Species:
- Human
- Sample Type:
- Plasma (mycoplasma pneumoniae pneumonia patients)
- Year:
- 2021
- Journal Name:
- Journal of Inflammation Research
- PubMed ID:
- 34429628
NLRP2 Regulates Proinflammatory and Antiapoptotic Responses in Proximal Tubular Epithelial Cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (proximal tubular epithelial cells)
- Year:
- 2019
- Journal Name:
- Frontiers in Cell and Developmental Biology
- PubMed ID:
- 31709256
Stretch-induced human myometrial cytokines enhance immune cell recruitment via endothelial activation
- Species:
- Human
- Sample Type:
- Conditioned Media (Stretch induced hTERT-HM cells)
- Year:
- 2014
- Journal Name:
- Cellular and Molecular Immunology
Myeloproliferative neoplasia remodels the endosteal bone marrow niche into a self-reinforcing leukemic niche.
- Species:
- Human
- Sample Type:
- Conditioned Media (Myeloid-enriched BM cells)
- Year:
- 2013
- Journal Name:
- Cell Stem Cell
- PubMed ID:
- 23850243
Prolonged circulation and in vivo efficacy of recombinant human granulocyte colony-stimulating factor encapsulated in polymeric micelles.
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2011
- Journal Name:
- Journal of Controlled Release
- PubMed ID:
- 21723892
Chemical synthesis and improved expression of recombinant human granulocyte colony-stimulating factor cDNA.
- Species:
- Human
- Sample Type:
- Proteins (protein expression in e. coli)
- Year:
- 2011
- Journal Name:
- Genetics and Molecular Research
- PubMed ID:
- 22057963
Functional expression cloning of molecules inducing CD34 expression in bone marrow-derived stromal myofibroblasts
- Species:
- Human
- Sample Type:
- Conditioned Media (nFibs)
- Year:
- 2020
- Journal Name:
- Biochemical and Biophysical Research Communications
Enteral Granulocyte-Colony Stimulating Factor and Erythropoietin Early in Life Improves Feeding Tolerance in Preterm Infants: A Randomized Controlled Trial
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2014
- Journal Name:
- The Journal of Pediatrics
