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Human Angiogenin ELISA Kit
Human Angiogenin ELISA Kit
RayBio® Human Angiogenin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1.5 pg/ml - 400 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 1,000 - 10,000 fold |
| Accession Number | P03950 |
| Gene Id | 283 |
| Gene Symbols | ANG, RNASE5 |
| Protein Name / Synonyms | Angiogenin (EC 3.1.27.-) (Ribonuclease 5) (RNase 5) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human Angiogenin qIPCR ELISA Kit
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RayPlex® Human Angiogenin Bead Assay
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Angiogenin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Angiogenin (ANG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage
- Species:
- Human
- Sample Type:
- Plasma; Extracellular Vesicles (extracellular vesicles EVs)
- Year:
- 2025
- Journal Name:
- Stroke and vascular neurology
- PubMed ID:
- 39357895
Secretome from estrogen-responding human placenta-derived mesenchymal stem cells rescues ovarian function and circadian rhythm in mice with cyclophosphamide-induced primary ovarian insufficiency
- Species:
- Human
- Sample Type:
- Other / Unspecified (CM, E2-CM)
- Year:
- 2024
- Journal Name:
- Journal of Biomedical Science
- PubMed ID:
- 39390588
Angiogenic properties of dehydrated human amnion/chorion allografts
- Species:
- Human
- Sample Type:
- Tissue Lysate (Dehydrated Human Amnion/Chorion Membrane Allografts)
- Year:
- 2014
- Journal Name:
- Vascular Cell
Cellular vascular endothelial growth factor and serum angiogenin in acute myeloid leukemia (clinical and prognostic significance).
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- The Egyptian Journal of Haematology
Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing
- Species:
- Human
- Sample Type:
- Conditioned Media (Amnion derived Multipotent cell suerpnate)
- Year:
- 2008
- Journal Name:
- Eplasty
Serum Angiopoietin Levels are Different in Acute and Chronic Myeloid Neoplasms: Angiopoietins do not only Regulate Tumor Angiogenesis
- Species:
- Human
- Sample Type:
- Serum (Newly diagnosed Myeloid neoplasia patients)
- Year:
- 2016
- Journal Name:
- Indian Journal of Hematology and Blood Transfusion
- PubMed ID:
- 27065577
