Human Adrenocorticotropic Hormone (ACTH) ELISA Kit
Referencia MBS267135-96
embalaje : 96-Strip-Wells
Marca : MyBiosource
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Teléfono : +1 850 650 7790
Adrenocorticotropic Hormone (ACTH) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS267135Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ACTH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
Adrenocorticotropic Hormone (ACTH), ELISA Kit
Full Product Name
Human Adrenocorticotropic Hormone (ACTH) ELISA Kit
Product Gene Name
ACTH elisa kit
Species Reactivity
Human
Assay Type
Sandwich
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ACTH concentration
Detection Range
1000 pg/ml-15.6 pg/ml
Sensitivity
5 pg/ml.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
26,707 Da
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of ACTH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ACTH purchase
MBS267135 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adrenocorticotropic Hormone (ACTH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTH. The ELISA analytical biochemical technique of the MBS267135 kit is based on ACTH antibody-ACTH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for ACTH. It may not necessarily be applicable to this product.
NCBI Official Full Name
adrenocorticotropic hormone, partial
NCBI Official Synonym Full Names
pro-opiomelanocortin-alpha
NCBI Official Symbol
Pomc
NCBI Official Synonym Symbols
BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
UniProt Protein Name
Pro-opiomelanocortin
UniProt Synonym Protein Names
Corticotropin-lipotropinCleaved into the following 10 chains:NPP; Melanotropin gammaAlternative name(s):Gamma-MSH
Protein Family
Corticotropin
UniProt Gene Name
Pomc
UniProt Synonym Gene Names
Pomc1; POMC; ACTH; CLIP
UniProt Entry Name
COLI_MOUSE
NCBI Protein Information
pro-opiomelanocortin; beta-endorphin; met-enkephalin; melanotropin gamma; corticotropin-lipotropin; adrenocorticotropic hormone; adrenal corticotropic hormone; alpha-melanocyte stimulating hormone; corticotropin-like intermediary peptide
NCBI Summary for ACTH
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Show more UniProt Comments for ACTH
POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; peroxisomal matrix; cytoplasm; extracellular region; secretory granule
Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; type 4 melanocortin receptor binding; hormone activity; receptor binding
Biological Process: generation of precursor metabolites and energy; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; signal transduction; glucose homeostasis; cellular pigmentation
Show more Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; peroxisomal matrix; cytoplasm; extracellular region; secretory granule
Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; type 4 melanocortin receptor binding; hormone activity; receptor binding
Biological Process: generation of precursor metabolites and energy; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; signal transduction; glucose homeostasis; cellular pigmentation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with ACTH elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Androgen Biosynthesis Pathway antibodies | Androgen Biosynthesis Pathway Diagram |
| Biological Oxidations Pathway antibodies | Biological Oxidations Pathway Diagram |
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| Cytochrome P450 - Arranged By Substrate Type Pathway antibodies | Cytochrome P450 - Arranged By Substrate Type Pathway Diagram |
| Endogenous Sterols Pathway antibodies | Endogenous Sterols Pathway Diagram |
| G Alpha (i) Signalling Events Pathway antibodies | G Alpha (i) Signalling Events Pathway Diagram |
| G Alpha (s) Signalling Events Pathway antibodies | G Alpha (s) Signalling Events Pathway Diagram |
| G-protein Activation Pathway antibodies | G-protein Activation Pathway Diagram |
Organs/Tissues associated with ACTH elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >18343 publications with ACTH and Brain |
| Pituitary Gland Antibodies | >8993 publications with ACTH and Pituitary Gland |
| Skin Antibodies | >1858 publications with ACTH and Skin |
| Testis Antibodies | >553 publications with ACTH and Testis |
| Embryonic Tissue Antibodies | >390 publications with ACTH and Embryonic Tissue |
| Uncharacterized Tissue Antibodies | >2 publications with ACTH and Uncharacterized Tissue |
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