HEK-293T cells
Referencia T0011002
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Origin:
Embryonic kidney Homo sapiens Kidney Epithelial Adherent transformed with Adeno and SV-40 viral DNA sequences DMEM + 2 mM Glutamine + 10% FBS 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C Complete culture medium supplemented with 5% (v/v) DMSO II Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Species:
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References for HEK-293T cells:
1. Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999.