G CSF Protein
Referencia OPPA00485
embalaje : 2ug
Marca : Aviva Systems Biology
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G CSF Protein (OPPA00485)
| Datasheets/Manuals | Printable datasheet for OPPA00485 |
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| Predicted Species Reactivity | Human |
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| Product Format | G-CSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Host | E. Coli |
| Additional Information | Solubility: It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| :: | Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 108 IU/mg. |
| :: | Protein Content: G-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GCSF as a Reference Standard. Protein Content: G-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GCSF as a Reference Standard. |
| :: | Product Introduction: GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes. Product Description: Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.G-CSF is purified by proprietary chromatographic techniques. |
| Reconstitution and Storage | Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined byAnalysis by RP-HPLC.Analysis by SDS-PAGE. |
| Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Met - Thr - Pro - Leu - Gly. |
| Gene Symbol | G CSF |
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| Alias Symbols | GCSF, CSF3OS, C17orf33 |
| NCBI Gene Id | 1440 |
| Protein Name | Granulocyte colony-stimulating factor |
| Description of Target | Recombinant Human Granulocyte-Colony Stimulating Factor |
| Uniprot ID | P09919 |
| Protein Accession # | NP_000750.1 |
| Protein Size (# AA) | Recombinant |
