Concanavalin A (Con A) (Cy3)

Cy3 can be used to viualize the binding pattern of Concanavalin A (Jackbean) Lectin (Con A) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling

Carbohydrate Specificity:
Glucose, Mannose

Inhibitory Carbohydrate:
a-Methylglucoside, a-Methylmannoside

Activity:
Relatively weak blood agglutinin. >10ug/ml will agglutination of neuraminidase-treated human erythrocytes.

Fluorescence:
Orange

Divalent Ions:
Ca++, Mn++

Excitation:
550nm

Emission:
570nm

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. CY3 conjugates are sensitive to light.