Chicken Interleukin 4,IL-4 ELISA Kit
Referencia CSB-E06756Ch-24T
embalaje : 24T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Interleukin 4,IL-4
Abbreviation
IL-4
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 86-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-100 | |||||
Recovery
| The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-97 | ||||
| EDTA plasma (n=4) | 92 | 85-96 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Chicken IL-4 ELISA Kit was designed for the quantitative measurement of Chicken IL-4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
Citations
- Evaluation of a Dendritic Cell–Targeted Chimeric EtIMP1 Vaccine Against Eimeria tenella in Chickens R Chen, M Mohsin, S Ma, S Su, L Wang, D Wang,Pakistan Veterinary Journal,2026
- T Yang, T Wang, G Zhang, Q Guo, Y Zhang, Y Gao,Poultry Science,2026
- XF Lin, XG Wang, CS Fu, ZS Zhang,Animals,2026
- H Kondo, S Iino, T Fukuda, M Aoki, Y Yoshimura,Poultry Science,2025
- S Younas, DA Bukhari, Z Bibi, A Ullah, A Rehman,Poultry Science,2025
- R Abbasi Kali, Iranian Journal of Veterinary Research,2023
- OJ Olarotimi,Olarotimi O J,,2023
- Zonghui Zuo,Scientific Reports,2021
- Rui Fang,Parasitology Research,2021
- Xin Liu,Italian Journal of Animal Science,2021
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