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> Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

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Referencia CSB-E09867Ch-24T

embalaje : 24T

Marca : Cusabio

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Teléfono : +1 850 650 7790

CSB-E09867Ch
Size 96T,5×96T,10×96T

Product Details

Associated names
IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Abbreviation
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
125 pg/mL-2000 pg/mL
Sensitivity
125 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-96  
1:2 Average % 97  
Range % 92-105  
1:4 Average % 87  
Range % 85-90  
1:8 Average % 96  
Range % 93-99  
Recovery
The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-94  
EDTA plasma (n=4) 95 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.183 0.194 0.189    
1000 0.311 0.333 0.322    
500 0.526 0.500 0.513    
250 1.024 1.028 1.026    
125 2.107 2.120 2.114    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Chicken IGF1 ELISA Kit was designed for the quantitative measurement of Chicken IGF1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-2000 pg/mL and the sensitivity is 125 pg/mL.

Citations

  • LA Vaccaro, K Herring, A Wilson, E England, AL Smith,Poultry Science,2024
  • G Cao,Poultry science,2024
  • VA Uyanga,Poultry science,2023
  • Q Chang,Poultry Science,2020
  • T. G. Wandita.et al,Tropical Animal Science Journal,2018

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Target Background

Function
(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are...
Gene References into Functions
  1. It is a protein synthesis-related factor.
  2. The coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken.
  3. The IGF-I gene is significantly associated with some growth traits (body weight at hatching, and at 4, 8, 12, and 14 weeks of age) in chickens.
  4. IGF1 single-nucleotide polymorphism c.47673G > A was associated with body weight and haematocrit percentage, and also with feed intake and percentages of abdominal fat and gizzard genotype x sex interactions.
  5. Insulin-like growth factor 1 stimulates Non-astrocytic Inner Retinal Glial cells to proliferate, migrate distally into the retina, and become reactive.
  6. The objective of this study was to examine associations of an IGF1 promoter polymorphism with growth, body composition, skeleton integrity, and metabolic traits in chickens.
  7. Genetic/nutritional models show a positive relation between muscle IGF-1 mRNA levels, which determine paracrine IGF-1 levels, and post hatch muscle growth. (Review)
  8. These results suggest that the mechanism of action whereby IGFBP2 excess impairs long bone development is to inhibit IGF-I and IGF-II mediated proliferation and matrix synthesis.
  9. Polymorphisms within the IGF1 gene are associated with 5-week body weight.
  10. Blood levels ofIGF-I may be more important than IGF-II after chicken hatching
  11. Data show that transcription level of IGF-1 decreased dramatically in the sex-linked dwarf.
  12. Association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken.
  13. IGF-1 genes and proteins are expressed at high or moderately high levels in healing tendon

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Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links

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