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> cGAMP [849214-04-6]

cGAMP [849214-04-6]

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Descripciòn

cGAMP (Cyclic GMP-AMP) functions as an endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA. cGAMP activates stimulator of interferon genes (STING), which activates a signaling cascade leading to the production of type I interferons and other immune mediators[1][2][3][4].

IC50 & Target

Endogenous Metabolite
Cellular Effect
Cell Line Type Value Description References
H69AR EC50
4.74 μM
Compound: 3',3'-cGAMP
Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay
Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay
[PMID: 34019405]
H69AR EC50
58.46 μM
Compound: 3',3'-cGAMP
Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay
Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay
[PMID: 34019405]
L929 EC50
20 nM
Compound: 3',3'-cGAMP
Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR
Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR
[PMID: 32206239]
PBMC EC50
>300 μM
Compound: 3',3'-cGAMP
Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs
Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs
[PMID: 34019405]
PBMC EC50
15.14 μM
Compound: 3',3'-cGAMP
Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs
Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs
[PMID: 34019405]
PBMC EC50
17.25 μM
Compound: 3'3'-cGAMP
Induction of IFNgamma secretion in human PBMC incubated for 16 hrs
Induction of IFNgamma secretion in human PBMC incubated for 16 hrs
[PMID: 36201304]
PBMC EC50
18.72 μM
Compound: 3'3'-cGAMP
Induction of TNFalpha secretion in human PBMC incubated for 16 hrs
Induction of TNFalpha secretion in human PBMC incubated for 16 hrs
[PMID: 36201304]
PBMC EC50
26.71 μM
Compound: 3',3'-cGAMP
Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs
Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs
[PMID: 34019405]
PBMC EC50
7.65 μM
Compound: 3'3'-cGAMP
Induction of IFNalpha secretion in human PBMC incubated for 16 hrs
Induction of IFNalpha secretion in human PBMC incubated for 16 hrs
[PMID: 36201304]
In Vitro

cGAMP promotes the antigen-specific proliferation capacity of spleen cells in mice[2].
cGAMP directly activates murine and human dendritic cells in vitro[2].
On stimulation with cGAMP, fibroblasts from the patients showed increased transcription of IFNB1 but not of the genes encoding interleukin-1 (IL1), interleukin-6 (IL6), or tumor necrosis factor (TNF)[3].
cGAMP activates the endoplasmic reticulum (ER)-resident receptor STING, thereby inducing an antiviral state and the secretion of type I IFNs[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

cGAMP Related Antibodies
In Vivo

cGAMP (5 μg; nostril mucosal adjuvant) promotes the antigen-specific cytokine production by spleen cells of immunized mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 (H-2b) mice 6–8 weeks old[2]
Dosage: 5 µg
Administration: Nostril mucosal adjuvant
Result: Higher titers of ovalbumin (OVA)-specific IgA and total IgG as well as IgG1 and IgG2c in the sera of mice immunized with cGAMP-adjuvanted OVA as compared to sera from OVA-immunized mice.
Peso molecular

674.41

Fòrmula

C20H24N10O13P2
No. CAS
Appearance

Solid

Color

White to yellow

SMILES

O=P(O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP4(O)=O)(O)OC[C@@H]5[C@@H](O4)[C@@H](O)[C@H](N6C=NC7=C6N=C(N)NC7=O)O5
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

H2O : 180 mg/mL (266.90 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4828 mL 7.4139 mL 14.8278 mL
5 mM 0.2966 mL 1.4828 mL 2.9656 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación
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