Cell Senescence β-Galactosidase Staining Kit is a kit for the detection of senescence-specific β-galactosidase (SA-β-Gal) in cells or frozen sections using X-gal as substrate. When cells sense, they express a special β-galactosidase, SA-β-Gal, which has high enzymatic activity at pH 6.0. At the right pH, SA-β-Gal can catalyze X-gal to produce the blue product. This product can be observed by ordinary light microscopy, and its content is positively correlated with the level of cellular senescence.

This kit stains only senescent cells, not presenescent cells, quiescent cells, immortalized cells, or tumor cells. This kit is optimized to be compatible with polystyrene consumables or containers such as common cell culture plates, pipettes, etc., which can effectively avoid poor staining results caused by incompatible consumables or containers. In addition, the working solution prepared by this kit will not produce precipitation during use, which is more convenient.

Figure 1: Staining of MCF-7 cells (A) and Etoposide-treated MCF-7 cells (B) with this kit The results showed that the kit only stained senescent cells, not normal cells