Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human lung cancer with CDK4 recombinant monoclonal antibody, clone R04-4I5 (Cat # RAB01514).
Western Blot
Western blot analysis of CDK4 in K562, C6, 3T3, Hela lysates using human CDK4 recombinant monoclonal antibody, clone R04-4I5 (Cat # RAB01514).
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against human Cdk4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human Cdk4
Theoretical MW (kDa)
Calculated MW: 34 kD
Reactivity
Human
Form
Liquid
Preparation Method
Mammalian cell (CHO) expression system
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1/100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human lung cancer with CDK4 recombinant monoclonal antibody, clone R04-4I5 (Cat # RAB01514).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunoprecipitation
Western Blot
Western blot analysis of CDK4 in K562, C6, 3T3, Hela lysates using human CDK4 recombinant monoclonal antibody, clone R04-4I5 (Cat # RAB01514). - Gene Info — CDK4
Entrez GeneID
1019Protein Accession#
P11802Gene Name
CDK4
Gene Alias
CMM3, MGC14458, PSK-J3
Gene Description
cyclin-dependent kinase 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq
Other Designations
cell division kinase 4|melanoma cutaneous malignant, 3
- Interactomes
- Pathways
- Diseases