Cdk1/Cdc2 (phospho-Y15) polyclonal antibody

Product Name :
Cdk1/Cdc2 (phospho-Y15) polyclonal antibody Background :
Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Cdk1/Cdc2 (Y15) polyclonal antibody detects endogenous levels of Cdk1/Cdc2 protein only when phosphorylated at Tyr15. Immunogen :
Synthetic phosphopeptide derived from human Cdk1/Cdc2 around the phosphorylation site of Serine 795. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of Cdc2 (phospho-Y15) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with UV (10min) whole cell lysate(40ug) Lane3:Hela treated with UV (20min) whole cell lysate(40ug) Lane4:Hela treated with UV (30min) whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of p-Cdk1/Cdc2 (Y15) pAb in paraffin-embedded human breast carcinoma tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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