CAT ELISA Kit (Dog)
Referencia OKEH03959
embalaje : 96Wells
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
CAT ELISA Kit (Dog) (OKEH03959)
| Datasheets/Manuals | Printable datasheet for CAT ELISA Kit (Dog) (OKEH03959) |
|---|
| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology CAT ELISA Kit (Dog) (OKEH03959) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAT captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.089 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Dog Catalase | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | CAT |
|---|---|
| Gene Full Name | catalase |
| Alias Symbols | catalase. |
| NCBI Gene Id | 403474 |
| Protein Name | Catalase |
| Description of Target | Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells. |
| Uniprot ID | O97492 |
| Protein Accession # | NP_001002984.1 |
| Nucleotide Accession # | NM_001002984.1 |
| Protein Size (# AA) | 527 |
| Molecular Weight | 527 kDa |
