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Canine IFN-gamma ELISA Kit
Canine IFN-gamma ELISA Kit
RayBio® Canine IFN-gamma ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Canine (Dog) |
| Sensitivity | 0.1 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.1 ng/ml - 25 ng/ml |
| Specificity | This ELISA antibody pair detects canine IFN-gamma, no cross-reactivity was observed with recombinant human, mouse, rat, cotton rat, feline, porcine, equine or bovine IFN-gamma. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P42161 |
| Gene Id | 403801 |
| Gene Symbols | IFNG |
| Protein Name / Synonyms | Interferon gamma (IFN-gamma) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Autophagy, Innate Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Canine IFN-gamma qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Canine IFN-gamma ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IFN-gamma (IFNG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Dietary yeast beta 1,3/1,6 glucan supplemented to adult Labrador Retrievers alters peripheral blood immune cell responses to vaccination challenge without affecting protective immunity
- Species:
- Canine
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Animal Science
A new combination of a prebiotic and postbiotic mitigates immunosenescence in vaccinated healthy senior dogs
- Species:
- Canine
- Sample Type:
- Serum (vaccinated senior dogs, D0/D28/D105)
- Year:
- 2024
- Journal Name:
- Frontiers in Veterinary Science
- PubMed ID:
- 39634761
Clinical and inflammatory response to antiviral treatments in dogs with parvoviral enteritis
- Species:
- Canine
- Sample Type:
- Serum (dogs with parvoviral enteritis)
- Year:
- 2024
- Journal Name:
- Journal of Veterinary Science
- PubMed ID:
- 38311324
Plasma Erythropoietin, IL-17A, and IFNγ as Potential Biomarkers of Motor Function Recovery in a Canine Model of Spinal Cord Injury
- Species:
- Canine
- Sample Type:
- Plasma (post-SCI time course)
- Year:
- 2020
- Journal Name:
- Journal of Molecular Neuroscience
- PubMed ID:
- 32418163
