Top: Standard Curve of Calcium Assay Kit (Cat # KA4081) with regular protocol. Calcium dose response was measured on a 96-well black plate with the Calcium Assay Kit (Cat # KA4081). As low as ~ 2.5uM Ca2+ was detected with 5 minutes incubation time (n=3).
Bottom: Standard Curve of the Calcium Assay Kit (Cat # KA4081) with protocol for serum or urine samples. Calcium dose response was measured on a 96-well black plate with the Calcium Assay Kit (Cat # KA4081). As low as 0.03 mM calcium was detected with 5 minutes incubation time (n=3).
- Specifications
Product Description
Calcium Assay Kit is used for the measurement of calcium level in physiology solutions.
Suitable Sample
Physiological Solutions
Sample Volume
50 uL
Absorbance (nm)
600 or 650 nm
Detection Method
Colorimetric
Instrument Platform
Microplate Reader
Calibration Range
0.047 - 1.5 mM
2.5 - 150 uMLimit of Detection
2.5 uM
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and minimize light exposure.
- Applications
Quantification
- Publication Reference
- Immunomodulation Using BMP-7 and IL-10 to Enhance the Mineralization Capacity of Bone Progenitor Cells in a Fracture Hematoma-Like Environment.
Matthias Guido Wiesli., Matthias Werner Huber., Bernhard Weisse., Robert Zboray., Stefanie Kiderlen., Arlyng González-Vázquez., Katharina Maniura-Weber., Markus Rottmar., William Arthur Lackington.
Advanced healthcare materials 2025 Feb; 14(5):e2400077.
Application：Calcium Assay, Human, CHA scaffolds, Human Bone Progenitor Cells.
- Influence of ceftriaxone on human bone cell viability and in vitro mineralization potential is concentration- and time-dependent.
Matthias Guido Wiesli, Jean-Pierre Kaiser, Emanuel Gautier, Peter Wick, Katharina Maniura-Weber, Markus Rottmar, Peter Wahl.
Bone & Joint Research 2021 Mar; 10(3):218.
Application：Func, Quant, Human, Human bone progenitor cells.
- Immunomodulation Using BMP-7 and IL-10 to Enhance the Mineralization Capacity of Bone Progenitor Cells in a Fracture Hematoma-Like Environment.