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> Anti-STRAD alpha Antibody

Anti-STRAD alpha Antibody

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Referencia CQA2461-200ul

embalaje : 200ul

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

  • Western blot analysis of STRAD alpha expression in Jurkat (A) whole cell lysates. (Predicted band size: 33; 34; 38; 41; 43; 48 kD; Observed band size: 48 kD)
  • Immunofluorescent analysis of STRAD alpha staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Flow cytometric analysis of A549 cells using Anti-STRAD alpha Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
Product Name:Anti-STRAD alpha Antibody
Cat No:CQA2461
Source:Rabbit
Reactivity:H
Applications:WB, IF/IC, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to STRAD alpha
Immunogen：Recombinant full length protein of human STRAD alpha
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of STRAD alpha protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), FC (1/10 - 1/30)
Gene Symbol：STRADA
Alternative Names：LYK5; STRAD; STE20-related kinase adapter protein alpha; STRAD alpha; STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96
Entrez Gene (Human)： 92335;
SwissProt (Human)： Q7RTN6;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.