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> Anti-PRKAR1B Antibody

Anti-PRKAR1B Antibody

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Referencia CPA1932-200ul

embalaje : 200ul

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

  • Western blot analysis of PRKAR1B expression in HEK293T (A), U2OS (B), MCF7 (C), mouse liver (D) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 55 kD)
  • Immunohistochemical analysis of PRKAR1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of PRKAR1B staining in PCCL cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blot analysis of PRKAR1B expression in A2780 (A), MG63 (B), Jurkat (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 55 kD)
Product Name:Anti-PRKAR1B Antibody
Cat No:CPA1932
Source:Rabbit
Reactivity:H, M, R, C, D, Z
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to PRKAR1B
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRKAR1B. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of PRKAR1B protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/50 - 1/200)
Gene Symbol：PRKAR1B
Alternative Names：cAMP-dependent protein kinase type I-beta regulatory subunit
Entrez Gene (Human)： 5575;
Entrez Gene (Mouse)： 19085;
SwissProt (Human)： P31321;
SwissProt (Mouse)： P12849;
SwissProt (Rat)： P81377;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.