Anti-CD49d Antibody-APC labled

Referencia CFB8536-100

embalaje : 100Assays

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

Product Name:Anti-CD49d Antibody-APC labled
Cat No:CFB8536
Source:Mouse
Reactivity:H
Applications:IF, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description:Mouse monoclonal antibody APC labled to CD49d
Immunogen:Native purified human CD49d.
Purification:The antibody was purified by affinity chromatography.
Specificity:Recognizes human CD49d
Clonality:Monoclonal (clone: BU49)
Conjugation:APC
Form:Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution:10 μl / assay
Gene Symbol:ITGA4
Alternative Names:CD49D; Integrin alpha-4; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD antigen CD49d
Entrez Gene (Human): 3676;
SwissProt (Human): P13612;
Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.