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> Anti-A-RAF (Phospho-Y302) Antibody

Anti-A-RAF (Phospho-Y302) Antibody

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Referencia CPA1072-50ul

embalaje : 50ul

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

  • Western blot analysis of A-RAF (Phospho-Y302) expression in HeLa PMA-treated (A), Raji (B), HT29 (C), NIH3T3 (D), SP2/0 PMA-treated (E), PC12 PMA-treated (F) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 68 kD)
  • Immunohistochemical analysis of A-RAF (Phospho-Y302) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of A-RAF (Phospho-Y302) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Product Name:Anti-A-RAF (Phospho-Y302) Antibody
Cat No:CPA1072
Source:Rabbit
Reactivity:H, M, R, B, P, S
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to A-RAF (Phospho-Y302)
Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y302 of human A-RAF protein. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of A-RAF protein only when phosphorylated at Y302.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)
Gene Symbol：ARAF
Alternative Names：ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
Entrez Gene (Human)： 369;
Entrez Gene (Mouse)： 11836;
Entrez Gene (Rat)： 64363;
SwissProt (Human)： P10398;
SwissProt (Mouse)： P04627;
SwissProt (Rat)： P14056;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.