Alizarin Red S sodium [130-22-3]
Referencia NB-64-05687-200mg
embalaje : 200mg
Marca : Neo Biotech
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Teléfono : +1 850 650 7790
Alizarin Red S sodium (Synonyms: ARS sodium)
Catalog No. T14181 Copy Product Info
Alizarin Red S sodium (ARS sodium) is an anthraquinone dye widely used to evaluate calcium deposits in cell culture[1].
Alizarin Red S sodium
Copy Product InfoSynonyms ARS sodium
Alizarin Red S sodium (ARS sodium) is an anthraquinone dye widely used to evaluate calcium deposits in cell culture[1].
Cas No. 130-22-3
Product Introduction
Alizarin Red S sodium AI Summary
Alizarin Red S sodium exhibits inhibitory activity against various protein tyrosine phosphatases, with IC50 values of 82000.0 nM for human Leukocyte Antigen Related (LAR) protein tyrosine phosphatase D1, 40000.0 nM for human PTPase 1B, and 48000.0 nM for Saccharomyces cerevisiae Tyrosine phosphatase 1. Additionally, it inhibits PGAM1 with an IC50 value of 2300.0 nM. In terms of kinase inhibition, Alizarin Red S sodium demonstrates potent activity against PKL, showing an IC50 value of 200.0 nM and complete inhibition at 10 µM in a luminescence assay, as well as displaying a 54.0% inhibition rate in human PKM2 knockout HepG2 cells at the same concentration. Furthermore, this compound demonstrates growth inhibition activity against various human tumor cell lines, with a GI50 value of 100000.0 nM for most cell lines. Notably, it shows slightly lower GI50 values against the SK-MEL-2 Melanoma, SF-539 Central Nervous System, and SK-MEL-28 Melanoma cell lines, suggesting higher potency against these cancers. These attributes indicate potential bioactivity related to regulating protein dephosphorylation processes and anti-cancer properties..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Alizarin Red S sodium (ARS sodium) is an anthraquinone dye widely used to evaluate calcium deposits in cell culture[1]. |
| Cell Research | Solution preparation Preparation of mother solution: Prepare a 2% Alizarin Red S sodium aqueous solution. Preparation of working solution: Use ammonium hydroxide to adjust the pH to 4.2, filter the solution with a 0.45 μm microfilter and store it in an amber bottle and re-filter the solution with a 0.22 μm microfilter before use. I. Detection of osteoblast differentiation by Alizarin Red S sodium Operation steps 1. Induce osteoblast differentiation: MC3T3-E1 cells were seeded on four-well plates at 1×105 cells per well and cultured in α-MEM (without ascorbic acid) containing 10% fetal bovine serum. Osteoblast differentiation was induced with osteogenic medium (OSM) containing ascorbic acid (50 μg/mL) and β-glycerophospholipids (10 mM). 2. Staining: MC3T3-E1 cells were washed with 1×PBS, fixed with 70% ethanol at room temperature for 10 min, rinsed with distilled water, and stained with Alizarin Red S for 10 min to detect calcium deposition in the matrix. 3. Data analysis: The area of the red area is proportional to osteoblast differentiation. 2. Screening test of calcium compounds in synovial fluid by Alizarin Red S sodium 1. Synovial fluid preparation: Aspirate the synovial fluid into a disposable syringe, and then transfer the fluid to a clean test tube containing sodium heparin anticoagulant; 2. Staining: Aspirate a drop of synovial fluid from the bottom of the test tube, place it on a clean slide, mix it with a drop of 2% Alizarin Red S, and cover it with a coverslip; c3 Within three minutes after mixing, observe it under an ordinary optical microscope, and grade the degree of orange-red staining density observed in each high-power field of view. The area of the red area is proportional to the content of calcium compounds. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | ARS sodium |
| Molecular Weight | 342.26 |
| Formula | C14H7NaO7S |
| Cas No. | 130-22-3 |
| Smiles | [Na+].Oc1c(O)c2C(=O)c3ccccc3C(=O)c2cc1S([O-])(=O)=O |
| Relative Density. | no data available |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 41.67 mg/mL (121.75 mM), Sonication is recommended.  H2O: 10 mg/mL (29.22 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||