ADAPT-3D™ 5X Wash Buffer

ADAPT3D™ 5X Wash Buffer Components (at 1X working concentration):

PhosphateBuffered Saline (PBS): Provides isotonic conditions to maintain tissue structure

1.5 M Glycine: Quenches free aldehydes to reduce background fluorescence

5 U/mL Heparin: Minimizes nonspecific binding, particularly of antibodies and charged molecules

0.09% Sodium Azide: Preservative to prevent microbial contamination during long incubations

Store this 5X wash buffer at room temperature unless otherwise specified.

Room Temperature (RT) via Ground or 2Day Express Shipping

The ADAPT3D™ 5X Wash Buffer is an essential component of the ADAPT3D™ Tissue Clearing Kit, formulated to gently and effectively remove unbound reagents, antibodies, and residual clearing agents between protocol steps. Optimized for use with cleared tissues, this buffer maintains tissue integrity and fluorescence stability while supporting consistent and reproducible immunolabeling and imaging results. It is provided as a 5X concentrate for convenient dilution and efficient workflow integration.

The ADAPT3D™ 5X Wash Buffer is diluted to 1X and used throughout the protocol to thoroughly rinse tissue samples between key steps, including after delipidation and immunostaining. Its formulation helps remove unbound reagents and antibodies, reduce background fluorescence, and preserve tissue integrity and antigenicity during the clearing and labeling process. Multiple washes in this buffer help ensure optimal imaging clarity and consistency. Please review the ADAPT3D Protocol for more details.

This product contains sodium azide (0.09%), which is toxic if ingested, inhaled, or absorbed through the skin. Use appropriate personal protective equipment, including gloves, lab coat, and eye protection. Handle in a wellventilated area and dispose of in accordance with local regulations. For detailed safety and handling instructions, please refer to the Safety Data Sheet (SDS).

At Leinco Technologies, a U.S.based manufacturer located in the St. Louis, Missouri metropolitan area, we are committed to supporting life science research and diagnostics through the production of highquality blocking and assay reagents. All products are manufactured in our ISO 13485certified facility, ensuring they meet the highest standards of quality, consistency, and performance for use in a wide range of immunoassay and imaging applications.