embalaje : 1mLx10mM(inDMSO)
Synonyms: 6-Carboxyfluorescein diacetate
| Description | 6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption. |
| In vitro | Sperm plasma membrane integrity analysis Procedure: 1. Wash cryopreserved sperm and fresh sperm 3 times by centrifugation in PBS preheated at 36°C (3000 rpm × 5 min). 2. After washing, resuspend sperm in 1 mL PBS, mix with 10 μM PI and 1 μM 6-CFDA dye, and incubate at 37°C for 15 minutes. 3. After incubation, wash sperm 3 times with PBS and obtain images under fluorescence microscope. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | 6-Carboxyfluorescein diacetate |
| Molecular Weight | 460.3891 |
| Formula | C25H16O9 |
| Cas No. | 3348-03-6 |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=C(O2)C=C(OC(C)=O)C=C1 |
| Relative Density. | 1.57 g/cm3 (Predicted) |
| Storage | Keep away from direct sunlight, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 97.00 mg/mL (210.69 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.30 mg/mL (7.17 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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