Optimize Cell & Tissue Handling from Culture to Storage

Chick Embryo Extract (CEE)

• Cell culture supplement used in selected growth media formulations.
• Source of essential growth factors for specialized cell culture applications.
• Available as lyophilized* or frozen liquid formulations.
• Supports the in vitro cultivation of rat neural crest stem cells (NCSCs) and other neural explants.
• Used for the expansion of selected stem cell types and investigated for its role in DNA demethylation.
• Produced from chick embryos (Bovans Goldline) sourced from registered flocks free from clinical signs of notifiable disease.
• Typically supplied with penicillin, streptomycin, and amphotericin.
• Sterility tested for bacteria, yeast, and fungi.
• Growth-promotion tested using mouse muscle cell growth and differentiation assays.
• Quality-assured: batch-specific results are provided on the Certificate of Analysis.

* Please note that during the lyophilisation process the material is uncapped so sterility cannot be assured.

T-Store® Transport Media

• Chemically defined, optimized medium for the storage and transport of mammalian tissues and cells.
• Mimics the basic composition of interstitial fluid, helping maintain tissue pH and homeostasis during storage.
• Supports tissue integrity, helping limit necrosis and apoptosis.
• Suitable for up to 72 h of tissue storage and transport while retaining morphological and molecular profiles.
• Broad tissue compatibility, including normal and malignant tissues.
• Stable pH buffering: pH 7.15–8.20 across +2°C to +37°C.
• Compatible with aerobic or anaerobic conditions.
• Xeno-free and DMSO-free, with no serum or animal/human proteins.
• Endotoxin level: ≤1.0 EU/mL.
• Aseptically produced and sterility tested; 0.2 µm filtered.
• With antibiotics: shelf life reduced to 1 month from antibiotic addition.
• Applications: tissue collection, transport and storage prior to histological, molecular, or other downstream analyses; storage of isolated or enriched cells before further processing.

CellShip® Transport Media

• Alternative to cryopreservation for mammalian cell transport and short-term storage.
• Ready-to-use, sterile, defined, xeno-free formulation with no serum or animal/human proteins.
• DMSO-free and contains a non-toxic additive to help protect cells from shear stress and maintain membrane integrity.
• Validated for up to 120 h of transport/storage at ambient temperature for selected cell lines and 72 h for primary canine MSCs.
• Minimal cell manipulation, helping streamline handling and reduce contamination risk.
• Logistical alternative to dry-ice shipment, potentially simplifying time-critical workflows.
• Endotoxin level: ≤1.0 EU/mL.
• Manufactured under ISO 13485 standards.
• Sterile-filtered: 0.2 µm.
• Applications: transport and short-term storage of mammalian cells; ongoing evaluation for additional primary cells, stable cell lines, and gene-silenced cell lines.

Figure 1. Following assessment of T-Store® functionality after being stored at -20oC for 12 months. Mean fold change for cells following 72 h at ambient was 0.91, following the 48-h recovery period the mean fold change in viable cell number was 1.1. Mean cell viability following storage was 99% and following the recovery period mean viability was 93% (n=5).                              

Figure 2. HEK293F cells were stored in T-Store® for 72 h at room temperature, followed by 48 h recovery under standard culture conditions. Cell viability and recovery were assessed before and after storage, with fold change >1 defined as a successful functional outcome.                                                                                                                

Figure 3. Jurkat cells transported/stored in CellShip® at ambient had returned to pre-transport numbers within 24 h of recovery and showed a 2-fold increase by 48 h. Cell viability was well maintained throughout the experiment (n=3).                    

Figure 4. HepG2 cells were imaged following a 48-h recovery period. Prior to recovery, cells had been transported/stored for 72 h either cryopreserved in dry ice or at ambient in CellShip®. Within 48 h of recovery, cells transported in CellShip® were fully adherent and were displaying typical growth morphology (A & B). Cells recovered from cryopreservation had started to adhere but had not yet re-established typical HepG2 growth morphology (C & D).