Antibody arrays on glass slides are multiplex immunoassays that enable the parallel detection of multiple proteins in biological samples. In veterinary, comparative and translational research, arrays designed for species such as horses, sheep, rabbits, pigs, dogs and cats can support the investigation of immune responses and protein expression profiles. Depending on the array, targets may include cytokines, chemokines, growth factors and other immune-related proteins.
Key Features
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Multiplex protein detection: Simultaneous analysis of multiple analytes using spatially immobilized capture antibodies.
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Quantitative and semi-quantitative formats: Quantitative arrays use calibration standards and standard curves to estimate analyte concentrations within the validated measurement range. Semi-quantitative arrays generally compare relative signal intensities between samples or experimental conditions.
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Fluorescence-based detection: Many glass-slide antibody arrays use fluorescent detection and require a compatible microarray scanner and image-analysis software.
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Species- and analyte-dependent validation: Antibody specificity, cross-reactivity, sensitivity and assay performance depend on the species, target proteins, sample matrix and assay configuration.
Usual Research Applications
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Profiling cytokines and inflammatory mediators in veterinary and comparative immunology.
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Investigating host–pathogen interactions and species-specific immune responses.
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Comparing protein expression profiles across experimental conditions, disease states or treatment groups.
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Exploring candidate biomarkers and immune-related biological pathways in discovery research.
