WST-1  [150849-52-8]

Biological Activity

WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm.

Chemical Information

Molecular Weight	651.34
Formula	C19H11IN5NaO8S2
CAS Number	150849-52-8
Form	Solid
Solubility (25°C)	DMSO 25 mg/mL (ultrasonic and warming and heat to 60°C)
Storage	4°C, protect from light, dry, sealed

References

[1] Anika Bluemke, et al. Int J Cosmet Sci. Multidirectional activity of bakuchiol against cellular mechanisms of facial ageing - Experimental evidence for a holistic treatment approach

[2] Eleonora Scarcello, et al. PLoS One. Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese

[3] Gizem Calibasi-Kocal, et al. J BUON. Curcumin effects on cell proliferation, angiogenesis and metastasis in colorectal cancer

[4] Anne-Helen Lutter, et al. Clin Hemorheol Microcirc. Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures

[5] Puriya Ngamwongsatit, et al. J Microbiol Methods. WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line