Select Conjugation CF® 488A CF® 568 CF® 594 CF® 640R CF® 680 CF® 740 CF® 790 Biotin
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Staining of human and rat brain sections with CF® 488A Ulex Europaeus Agglutinin I (UEA-I). Human cerebral cortex or rat brain cryosections were fixed with PFA, permeabilized with Triton® X-100, then either left unstained or stained with 5 ug/mL CF® 488A UEA-I conjugate (green) in PBS for 15 minutes at RT, then rinsed in PBS. Human brain sections were mounted in EverBrite™ TrueBlack® Hardset Mounting Medium with NucSpot® 640 (nuclei, magenta), and rat brain sections were mounted in EverBrite™ Hardset Mounting Medium with NucSpot® 640 (nuclei, magenta). The UEA-I staining pattern was consistent with its reported localization to cerebral blood vessels in human brain, but UEA-I conjugate did not show specific staining in rat brain. Scale bars: 50 um. Staining of fixed and permeabilized HeLa cells with CF® 488A Ulex Europaeus Agglutinin I (UEA-I). PFA-fixed HeLa cells were permeabilized with PBS/0.1% Triton® X-100 for 10 minutes at RT, rinsed with PBS, stained with 5 ug/mL CF® 488A UEA-I conjugate (green) in PBS for 30 minutes, and then rinsed in PBS and imaged. In fixed and permeabilized HeLa cells, UEA-I predominantly stained intracellular membranes. PFA-fixed MCF7 cells stained with 5 ug/mL CF® 488A UEA-I conjugate (green); nuclei are stained with NucSpot® Live 650 (magenta). HeLa EVs were stained with Ulex Europaeus Agglutinin I (UAE I) CF® 488A conjugate with or without ExoBrite™ EV Stain Enhancer. The Enhancer reduces dye aggregates and increases the number of detected stained EVs, for improved signal-to-noise. View Spectra Viewer
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Product Description
Ulex europaeus agglutinin I (UEA I) binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. UEA I interacts specifically with blood group H type 2 antigen, which is expressed in individuals with type O blood and, to a lesser degree, in individuals with type A or B blood. This lectin is an established marker for human endothelial cells and has been used to differentiate between angiosarcomas and epithelial tumors. UEA I is also a marker for incompletely differentiated gastrin cells. The lectin may be used to stain tissue sections. Biotium offers UEA I conjugated to biotin and a selection of bright and photostable CF® Dyes.
Marker for human endothelial cells and incompletely differentiated gastrin cells
Binds to glycoproteins and glycolipids containing α-linked fucose residues
Suitable for immunofluorescence staining in tissue sections
Choice of 7 CF® Dyes or biotin
Supplied at 1 mg/mL in 10 mM HEPES pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2
Note: CF® Dye UEA I conjugates are formulated with sodium azide and are not suitable for use in vivo or live cell culture
Find the Right Stain for Your Application UEA I and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Datura stramonium lectin (DSL), Lycopersicon esculentum (Tomato), Phaseolus vulgaris leucoagglutinin (PHA-L), Wheat Germ Agglutinin (WGA) conjugates, Concanavalin A (Con A) and Arachis hypogaea (PNA) conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to other fluorescent dyes. Learn more about CF® Dyes.
Ulex Europaeus Agglutinin I (UEA I) Conjugates
Conjugation Ex/Em Size Catalog No. Dye Features
CF® 488A 490/516 nm 1 mL 29109 CF® 488A Features
CF® 568 562/584 nm 1 mL 29110 CF® 568 Features
CF® 594 593/615 nm 1 mL 29111 CF® 594 Features
CF® 640R 642/663 nm 1 mL 29112 CF® 640R Features
CF® 680 681/698 nm 1 mL 29113 CF® 680 Features
CF® 740 742/767 nm 1 mL 29133 CF® 740 Features
CF® 790 783/808 nm 1 mL 29148 CF® 790 Features
Biotin N/A 1 mL 29108
Full List of Lectin Conjugates
Product Features
CF® Dye Concanavalin A (Con A) • Cell surface stain for yeast, fungi, and mammalian cells® Dyes
CF® Dye Wheat Germ Agglutinin (WGA) • Cell surface stain for mammalian cells and gram+ bacteria® Dyes or HRP
CF® Dye Peanut Lectin (PNA) from Arachis hypogaea • Specific for terminal β-galactose and binds preferentially to galactosyl (β-1,3) N-acetylgalactosamine® dye colors
CF® Dye Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) • Marker for blood vessels and microglial cells® Dyes or biotin
CF® Dye Ulex Europaeus Agglutinin I (UEA I) • Marker for human endothelial cells and incompletely differentiated gastrin cells® Dyes or biotin
CF® Dye Phaseolus Vulgaris Leucoagglutinin (PHA-L) • Used to stimulate lymphocyte and T cell proliferation® Dyes or biotin
CF® Dye Datura Stramonium Lectin (DSL) • Binds to (beta-1,4) linked N-acetylglucosamine oligomers® Dyes or biotin
CF® Dye Sambucus Nigra Lectin (SNA, EBL)) • Binds to sialic acid attached to terminal galactose® Dyes or biotin
CF is a registered trademark of Biotium, Inc.
Tech Tips
Tech Tip: Five Steps to Successful Cell Membrane Staining & Imaging
Tech Tip: Guide for Selecting the Right Cell Surface Stain
Tech Tip: Workflows for Post-fixation Staining of Cells with Fluorescent Lectins
Product Attributes
Probe cellular localization
Membrane/cell surface
For live or fixed cells
For fixed cells, For live/intact cells
Cell permeability
Membrane impermeant
Fixation options
Fix before staining (formaldehyde), Fix after staining (formaldehyde), Fix before staining (methanol), Fix after staining (methanol), Permeabilize after staining
Colors
Green, Red, Far-red, Near-infrared
Documents, Protocols, SDS and COA
Datasheet
Datasheet Download
COA Request the Certificate of Analysis
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