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> Ubiquitin (UBB) Rabbit Polyclonal Antibody

Ubiquitin (UBB) Rabbit Polyclonal Antibody

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Referencia AP08123PU-N

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Ubiquitin (UBB) Rabbit Polyclonal Antibody

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SKU
AP08123PU-N
Ubiquitin (UBB) rabbit polyclonal antibody, Purified
Size:
1 ml 500 ul
Conjugation:
Unconjugated
 

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Specifications
Product Data
Application IF, IHC
Recommended Dilution Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system. FFPE tissue sections require antigen retriever, boiling tissue for 15-20 minutes in 10 mM citrate, pH 6.0, followed by cooling at RT for 15-20 minutes.
Immunofluorescence.
Recommended Positive Control: Human brain from Alzheimer's disease.
Reactivity Bovine, Equine, Human, Mouse, Rat
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Purified Human Ubiquitin.
Specificity This antibody stains senile plaques and neurons.
Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.
Buffer PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Protein A Chromatography
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Gene Name ubiquitin B
Database Link
Background Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin; it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer’s disease, lewy bodies in Parkinson’s disease, pick bodies in Pick’s disease, inclusions in motor neuron disease, Mallory’s Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes.
Synonyms UBB, Polyubiquitin-B
Reference Data
Protein Categories Intracellular Proteins
Product Manuals
FAQs
  • Antibody and Western Standard
SDS
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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