embalaje : 5mg
Teléfono : +1 850 650 7790
Synonyms: PG490, NSC 163062
| Description | Triptolide (PG490) belongs to the tricyclic diterpenoid group of natural products and is an inhibitor of NF-κB activation. Triptolide exhibits immunosuppressive, anti-rheumatic, anti-inflammatory, anti-proliferative and anti-tumor activities. |
| Targets & IC50 | MDM2-p53:47-73 nM (IC50) |
| In vitro | METHODS: Neuroblastoma cells BE(2)-C were treated with Triptolide (5-100 nM) for 24 h. Cell viability was measured using the CCK-8 Assay. RESULTS: A concentration-dependent response to Triptolide was observed in BE(2)-C cells. At 50 nM Triptolide, cell viability was significantly reduced to 50%. [1] METHODS: Breast cancer cells MCF-7 were treated with Triptolide (10-50 nmol/L) for 24 h, and the expression levels of target proteins were detected using Western Blot. RESULTS: Triptolide decreased the messenger RNA and protein levels of ERα in MCF-7 cells in a dose-dependent manner. [2] |
| In vivo | METHODS: To assay antitumor activity in vivo, Triptolide (0.4 mg/kg) was administered by gavage to BALB/c-nu+/nu+ mice harboring the breast cancer tumor MCF-7 once daily for three weeks. RESULTS: Triptolide inhibited the growth of MCF-7 cell xenografts in a mouse model. [2] METHODS: To investigate the effects on ulcerative colitis (UC), Triptolide (0.1-0.4 mg/kg) was administered orally to DSS-induced UC mice once daily for seven days. RESULTS: Triptolide has anti-inflammatory and therapeutic effects on UC mice. [3] |
| Synonyms | PG490, NSC 163062 |
| Cell Research | Triptolide is dissolved in DMSO (1 mg/mL) and stored, and then diluted with RPMI 1640 medium before use[3]. The viability of differentiated PC12 cells treated with different concentrations of Triptolide. After differentiated PC12 cells are cultured on 96-well plates with RPMI 1640 medium for stabilization, differentiated PC12 cells are incubated with different concentrations of Triptolide (0.01, 0.1, and 1 nM) for 24 hours. The concentrations in this study are chosen. Then cell viability is determined by the MTT assay. Each condition and experiment is repeated three times[3]. |
| Molecular Weight | 360.40 |
| Formula | C20H24O6 |
| Cas No. | 38748-32-2 |
| Smiles | 00[C@@]12C[C@@H]3O[C@@]33[C@H](O)[C@]4(O[C@H]4[C@@H]4O[C@]34[C@@]1(C)CCC1=C2COC1=O)C(C)C |
| Relative Density. | 1.48 g/cm3 |
| Storage | Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 51.00 mg/mL (141.51 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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