TPO, Recombinant, Human, Animal Free (Thrombopoietin, MGDF, Megakaryocyte Colony-Stimulating Factor, c-MPL Ligand)

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174aa polypeptide 18.6kD, which contains the erythropoietin-like domain of the full length TPO protein.

Manufactured using all Animal-Free reagents.

Recombinant protein corresponding to human Thrombopoietin, expressed in E. coli

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1ng/ml, corresponding to a specific activity of ≥1x10e6 units/mg.

Amino Acid Sequence:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.