Total Antioxidant Status (TAS) Colorimetric Assay Kit

Referencia E-BC-K801-M-500

embalaje : 500Assays

Marca : Elabscience

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Ready-to-Use Dual-Reagent

Total Antioxidant Status (TAS) Colorimetric Assay Kit (E-BC-K801-M)

Total Antioxidant Status (TAS) Colorimetric Assay Kit - 1
  • Total Antioxidant Status (TAS) Colorimetric Assay Kit - 1
  • Total Antioxidant Status (TAS) Colorimetric Assay Kit - 2
  • Total Antioxidant Status (TAS) Colorimetric Assay Kit - 3
All Size Price Qty
96T $ 150.00
- +
48T $ 100.00
- +

For research use only.

Detection Principle ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be reduced to colorless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status.
Synonyms TAS
Sample Type Serum,Plasma,Urine,Cellular supernatant,Animal tissue,Plant tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (650-670 nm)
Research Area Oxidative Stress
Other Reagents Required 60% Ethanol
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.23 mmol Trolox Equiv./L
Detection Range 0.23-2 mmol Trolox Equiv./L
Precision inter-assay CV: 7% | intra-assay CV: 4.6%
Sample Volume 10 μL
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K136-M Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit 96T , 48T
E-BC-K219-M Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method) 96T , 48T
E-BC-K225-M Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method) 500Assays , 96T , 48T
E-BC-K802-M Total Oxidant Status (TOS) Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Mouse liver tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat lung tissue homogenate 1
Molt4 cellar supernatant 1
Human urine 8-10
Mouse serum 1
Human serum 1
Human saliva 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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