TGFBI (NM_000358) Human Untagged Clone
Referencia SC119927
embalaje : 10ug
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TGFBI (NM_000358) Human Untagged Clone
SKU
SC119927
TGFBI (untagged)-Human transforming growth factor, beta-induced, 68kDa (TGFBI)
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | TGFBI |
| Synonyms | BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_000358 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@quimigen.pt or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000358.1, NP_000349.1 |
| RefSeq Size | 2691 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 7045 |
| UniProt ID | Q15582 |
| Cytogenetics | 5q31.1 |
| Domains | FAS1 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Summary | This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. provided by RefSeq, Jul 2008 |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC200411 | TGFBI (Myc-DDK-tagged)-Human transforming growth factor, beta-induced, 68kDa (TGFBI) | 10 ug | | |
| RC200411L1 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), Myc-DDK-tagged | 10 ug | | |
| RC200411L2 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), mGFP tagged | 10 ug | | |
| RC200411L3 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), Myc-DDK-tagged | 10 ug | | |
| RC200411L4 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), mGFP tagged | 10 ug | | |
| RG200411 | TGFBI (tGFP-tagged) - Human transforming growth factor, beta-induced, 68kDa (TGFBI) | 10 ug | | |
Citations
Dysregulation of the MiR-449b target TGFBI alters the TGFβ pathway to induce cisplatin resistance in nasopharyngeal carcinoma
Bissey, Law, Bruce et al
Oncogenesis (2018) 7 (5), 40 DOI: 10.1038/s41389-018-0050-x
Bissey, Law, Bruce et al
Oncogenesis (2018) 7 (5), 40 DOI: 10.1038/s41389-018-0050-x
AI Summary: OriGene products pCMV6-TGFBI-Myc and pCMV6-XL5-TGFBI (non-tagged) were used in this study to overexpress TGFBI in cell lines, enabling the investigation of TGFBI’s role in cisplatin resistance in nasopharyngeal carcinoma.
Snippet: were performed using calcium phosphate. JetPRIME (Polyplus-transfection, Graffenstaden, France) was used to transfect C666-1 for cell death assays and functional analysis in HEK293T. pCMV6-TGFBI-Myc (Origene, Rockville, MD, USA), pCMV6-XL5-TGFBI non-tagged (Origene), pcDNA3.1-beta-3 (Addgene plasmid #27289;from Timothy Springer, Addgene, Cambridge, MA, USA), pCX-EGFP beta5ΔC (Addgene plasmid #14;....
Four and a half domain 2 (FHL2) scaffolding protein is a marker of connective tissues of developing digits and regulates fibrogenic differentiation of limb mesodermal progenitors
Lorda-Diez, Montero, Sanchez-Fernandez et al
J Tissue Eng Regen Med (2018)
Lorda-Diez, Montero, Sanchez-Fernandez et al
J Tissue Eng Regen Med (2018)
AI Summary: In this study, the human βig-h3 (hs-βig-h3) cloned into the pCMV6-XL5 vector from OriGene was used for gain-of-function experiments to investigate the role of FHL2 in the fibrogenic differentiation of limb mesodermal progenitors.
Snippet: 2.5 Cell nucleofection. For gain-of-function experiments, we used Gallus gallus Fhl2 coding sequence (gg-Fhl2), G. gallus Scleraxis (gg-Sclx) cloned into the pcDNA3.1+GFP vector (GenScript), and Human βig-h3 (hs-βig-h3) cloned into pCMV6-XL5 (OriGene);For gain-of-function experiments, we used Gallus gallus Fhl2 coding sequence (gg-Fhl2), Gallus gallus Scleraxis (gg-Sclx), cloned into the pcDNA3.1+...
In vitro amyloid aggregate forming ability of TGFBI mutants that cause corneal dystrophies
Yam, Wang, Jhanji et al
Invest. Ophthalmol. Vis. Sci. (2012) 53 (9), 5890-8 DOI: 10.1167/iovs.11-9068
Yam, Wang, Jhanji et al
Invest. Ophthalmol. Vis. Sci. (2012) 53 (9), 5890-8 DOI: 10.1167/iovs.11-9068
Cited by 31
AI Summary: OriGene’s pCMV6_TGFBI expression vector, containing wild-type and mutant TGFBI genes, was used to transfect HEK293 cells to study the aggregation properties of TGFBI mutants associated with corneal dystrophies in this paper.
Snippet: Introns. Expression Constructs and Mutagenesis. A C-terminal myc-FLAG-tagged full-length open reading frame clone of human TGFBI (NM_000,358.2), named pCMV6_TGFBI, was obtained from Origene (Rockville, MD). CD
Showing 1-3 of 3 papers.
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