TGFA ELISA Kit (Monkey)
Referencia OKBB00740
embalaje : 96wells/kit,withremovablestrips.
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
TGFA ELISA Kit (Monkey) (OKBB00740)
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
|---|
| Predicted Species Reactivity | Monkey | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva's Monkey TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey TGF alpha amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 15.6 pg/ml - 1,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
| ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||||||||||||||||||||||
| Additional Information | To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
*number of samples for each test n=16. | ||||||||||||||||||||||||||||||||||||||||||
| :: |
| ||||||||||||||||||||||||||||||||||||||||||
| Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate TGF alpha | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | <1 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V40-A89 | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | TGFA |
|---|---|
| Gene Full Name | Protransforming growth factor alpha |
| Alias Symbols | Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA; |
| NCBI Gene Id | 613031 |
| Protein Name | Protransforming growth factor alpha |
| Description of Target | Transforming growth factor alpha(TGF-alpha) is upregulated in some monkey primate cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to monkey primate chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic monkey primate TGF-alpha showed similar immunoreactivity when compared with monkey primate TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines. |
| Uniprot ID | P01135 |
| Protein Accession # | NP_001093161.1 |
| Nucleotide Accession # | NM_001099691.2 |