Request Quote or Bulk Order Product Description
CF® Dyes conjugates of Streptavidin are high quality biotin-binding proteins labeled with the superior CF® Dyes or a selection of other labels. These conjugates are typically used as secondary reagents to detect biotinylated probes such as primary antibodies for flow cytometry, western blotting, immunofluorescence staining, and other applications.
- Conjugates with wide choice of CF® Dye colors, plus HRP, R-PE, APC, PerCP, and Alkaline Phosphatase
- Superior CF® Dyes are bright, photostable, and water-soluble
- Ideal for flow cytometry, western blotting, and immunofluorescence staining
Blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets.
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Streptavidin Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog number |
| CF®350 Streptavidin | CF®350 | 347/448 nm | 1 mg | 29031 |
| CF®405S Streptavidin | CF®405S | 404/431 nm | 1 mg | 29032 |
| CF®405M Streptavidin | CF®405M | 408/452 nm | 1 mg | 29033 |
| CF®405L Streptavidin | CF®405L | 395/545 nm | 1 mg | 29056 |
| CF®430 Streptavidin | CF®430 | 426/498 nm | 1 mg | 29065 |
| CF®440 Streptavidin | CF®440 | 440/515 nm | 1 mg | 29066 |
| CF®488A Streptavidin | CF®488A | 490/515 nm | 1 mg | 29034 |
| CF®514 Streptavidin | CF®514 | 516/548 nm | 1 mg | 29081 |
| CF®532 Streptavidin | CF®532 | 527/558 nm | 1 mg | 29030 |
| CF®543 Streptavidin | CF®543 | 541/560 nm | 1 mg | 29043 |
| CF®555 Streptavidin | CF®555 | 555/565 nm | 1 mg | 29038 |
| CF®568 Streptavidin | CF®568 | 562/583 nm | 1 mg | 29035 |
| CF®583R Streptavidin | CF®583R | 586/609 nm | 1 mg | 29086 |
| CF®594 Streptavidin | CF®594 | 593/614 nm | 1 mg | 29036 |
| CF®633 Streptavidin | CF®633 | 630/650 nm | 1 mg | 29037 |
| CF®640R Streptavidin | CF®640R | 642/662 nm | 1 mg | 29041 |
| CF®647 Streptavidin | CF®647 | 650/665 nm | 1 mg | 29039 |
| CF®660R Streptavidin | CF®660R | 663/682 nm | 1 mg | 29040 |
| CF®680R Streptavidin | CF®680R | 680/701 nm | 1 mg | 29072 |
| CF®740 Streptavidin | CF®740 | 742/767 nm | 1 mg | 29129 |
Alkaline Phosphatase
Streptavidin (1 mg/mL) | Alkaline
Phosphatase | N/A | 100 uL | 29071-100uL |
| 1 mL | 29071-1mL |
| HRP Streptavidin | HRP | N/A | 1 mg | 29049 |
R-PE Streptavidin
(0.5 mg/mL) | R-PE | 496, 546, 565/578 nm | 200 uL | 29044-200uL |
| 1 mL | 29044-1mL |
APC Streptavidin
(0.5 mg/mL) | APC | 650/660 nm | 200 uL | 29048-200uL |
| 1 mL | 29048-1mL |
PerCP Streptavidin
(0.5 mg/mL) | PerCP | 482/675 nm | 200 uL | 29140-200uL |
| 1 mL | 29140-1mL |
Storage and Handling
Liquid formats:
Streptavidin, R-PE Conjugate and PerCP Conjugate: Store at 4°C, protected from light. Storage at -20°C is not recommended for R-PE or PerCP conjugates. Product is stable for at least 6 months from date of receipt when stored as recommended.
Streptavidin, APC Conjugate: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended.
Streptavidin, Alkaline Phosphatase Conjugate: Store at -20°C. Product is stable for at least 6 months from date of receipt when stored as recommended.
Lyophilized format:
CF® Dye Streptavidin Conjugates: Product is stable for at least 2 years at -20°C with desiccant. Add 0.5 mL of PBS to 1 mg lyophilized CF® Dye Streptavidin Conjugate and mix gently to dissolve.
Streptavidin, HRP Conjugate: add 1 mL pH ~7.4 1X PBS and mix gently to dissolve. Reconstituted HRP conjugate can be stored in aliquots at -20°C for at least 6 months. Do not add azide to HRP conjugates.
CF is a registered trademark of Biotium
Technology
CF® Dye & Other Bioconjugates
Tech Tips
Troubleshooting Tips for Fluorescence Staining
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Fluorescence Bleed-Through and Cross-Talk
Product Attributes
Colors
Blue, Green, Orange, Red, Far-red, Near-infrared
Detection method/readout
Fluorescence microscopy, Live cell imaging, Flow cytometry, Near-IR imager, In vivo near-IR imaging
Antibody reactivity (target)
Biotin
Antibody/conjugate formulation
AP conjugates: 1 mg/mL in 50 mM Tris buffer (pH 8.0) with 1 mM MgCl2 , 50% glycerol, and 0.05% sodium azide, APC Conjugate: 0.5 mg/mL in PBS, 50% glycerol, 2 mg/mL BSA (IgG-free and protease-free), and 0.05% sodium azide, CF® Dye Conjugates: 2 mg/mL in 1X PBS with 0.01% sodium azide after reconstitution, HRP conjugate: 1 mg/mL PBS/10 mg/mL trehalose after reconstitution, PerCP Conjugate: 0.5 mg/mL in PBS, 2 mg/mL BSA (IgG-free and protease-free), and 0.05% sodium azide, R-PE Conjugate: 0.5 mg/mL in PBS, 2 mg/mL BSA (IgG-free and protease-free), and 0.05% sodium azide
Product origin
Alkaline Phosphatase: Recombinant, E. coli, HRP: Horseradish (Armoracia rusticana), Streptavidin, recombinant from E. coli, APC from algae, BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells., PerCP from algae
Documents, Protocols, SDS and COA
Datasheet
Datasheet Download
COARequest the Certificate of Analysis
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