Stat1 (phospho-S727) polyclonal antibody

Product Name :
Stat1 (phospho-S727) polyclonal antibody Background :
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Stat1 (S727) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Ser727. Immunogen :
Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Serine 727. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of Stat1 (phospho-S727) polyclonal antibody at 1:500 dillution Lane1:NIH-3T3 whole cell lysate(40μg) Lane2:NIH-3T3 treated with UV(4h) whole cell lysate(40μg) Lane3:NIH-3T3 treated with PMA(60ng/ml, 30min) whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:Hela treated with IFN-γ(100ng/ml, 15min) whole cell lysate(40μg) Lane6:Hela treated with PMA(60ng/ml, 30min) whole cell lysate(40μg)
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  Immunohistochemistry (IHC) analyzes of p-Stat1 (S727) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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