Disease:

Neuroblastoma

Origin:

Brain; derived from metastatic site: bone marrow. The SK-N-SH line differs from SK-N-MC in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase.

Species:

Homo sapiens

Tissue:

Brain

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

2n = 46, modal no. 47

Patient:

Female, Caucasian, 4 years of age

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; seed at 3x10^4 cells/cm2, feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO