SetDB1, Chemiluminescent, BioAssay™ Kit (Histone-Lysine N Methyltransferase SETDB1, SET Domain Bifurcated 1, KMT1E, ERG-Associated Protein with SET Domain, ESET)

Intended Use:
The SetDB1 Chemiluminescent BioAssay™ Kit is designed to measure SetDB1 activity for screening and profiling applications.

Test Principle:
The key to the SetDB1 Direct Activity BioAssay™ Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Applications:
Great for studying enzyme kinetics and HTS applications.

Kit Components:
8-well strip plate module precoated with histone substrate, 12x8-well strips, 1x plate
SetDB1 human enzyme, 1x5ug
20uM S-adenosylmethionine, 1x250ul
Primary antibody 1, 1x100ul
Secondary HRP-labeled antibody 1, 1x10ul
4x HMT assay buffer 1, 1x3ml
Blocking buffer, 1x50ml
HRP chemiluminescent substrate A, (transparent bottle), 1x6ml
HRP chemiluminescent substrate B, (brown bottle), 1x6ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.