Cell cryopreservation is an important process for preserving cells. The best technique for cell cryopreservation is storing them in liquid nitrogen with an appropriate freezing medium. Cryopreserved cells can be recovered for culture when needed.

Serum-Free Cell Freezing Medium (HEK293 cells) is a sterile, serum-free and protein-free medium, which is suitable for the cryopreservation of HEK293 cells. This medium is ready-to-use, and does not need any additives, which is very easy to use.

This medium is chemically defined, containing nutrients such as glucose, amino acids, cryoprotectant dimethyl sulphoxide (DMSO) and phenol red. Meanwhile, this medium is FBS-free, protein-free, and animal origin-free, which can reduce cryopreservation differentiation and cell contamination caused by serum batch, and reduce the effect of exogenous protein on cell growth and differentiation. This medium has been demonstrated to result in high viability after freeze-thaw procedures (cell recovery rates >85%) that is comparable to cells preserved in FBS and DMSO.

Serum-free, protein-free

Ready-to-use, sterile medium

Maintenance of high viability after freeze-thaw procedures (cell recovery rates >85%)

Low risk of cell contamination, safer use

Simple freezing protocol