RPL35 polyclonal antibody

Product Name :
RPL35 polyclonal antibody Background :
ribosomal protein L35(RPL35) Homo sapiens Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008], Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ribosomal Protein L35 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L35 protein. Immunogen :
The antiserum was produced against synthesized peptide derived from human RPL35. AA range:51-100 Conjugate :
Unconjugated Modification :
Unmodification

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  Western Blot analysis of various cells using Ribosomal Protein L35 Polyclonal Antibody diluted at 1：2000
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  Western blot analysis of lysates from 293, HeLa, COLO, and Jurkat cells, using RPL35 Antibody. The lane on the right is blocked with the synthesized peptide.
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  Western blot analysis of lysates from 293, HeLa, COLO, and Jurkat cells, using RPL35 Antibody. The lane on the right is blocked with the synthesized peptide.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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