Recombinant SARS-CoV-2 Spike Protein, S2 Subunit derived from E.coli
Product Description
Specifications
| Size | 100 µg, 250 µg |
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Protein Name / Synonyms | SARS-CoV-2 Spike Protein, S2 Subunit, S protein, spike protein |
| Expressed Region | Met697 - Pro1213 |
| Expression System | E. coli |
| Tag | N-terminal histidine tag |
| Purity | >95 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | ∼58 kDa |
| Format | Liquid |
| Formulation | Filtered solution in 50 mM Tris-HCl, 500 mM NaCl, 600 mM arginine, 2 mM oxidized glutathione, 4 mM reduced glutathione, 0.2% PEG4000, 20% glycerol (pH 8.5). |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bradford protein assay. |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image

Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
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citations
Spike 1 protein of SARS-CoV-2 induces endothelial inflammation and vascular dysfunction through interferon ISG15-dependent mechanisms
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (endothelial cells)
- Year:
- 2026
- Journal Name:
- Cardiovascular Research
- PubMed ID:
- 42150851
SARS-CoV-2 Spike Protein Stimulates Macropinocytosis in Murine and Human Macrophages via PKC-NADPH Oxidase Signaling
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (human macrophages)
- Year:
- 2024
- Journal Name:
- Antioxidants
- PubMed ID:
- 38397773
Overcoming antibody-resistant SARS-CoV-2 variants with bispecific antibodies constructed using non-neutralizing antibodies
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (immunogen for BALB, c mice hybridoma production)
- Year:
- 2024
- Journal Name:
- iScience
- PubMed ID:
- 38500835
Understanding the Spike Protein in COVID-19 Vaccine in Recombinant Vesicular Stomatitis Virus (rVSV) Using Automated Capillary Western Blots
- Year:
- 2023
- Journal Name:
- ACS omega
Direct activation of the alternative complement pathway by SARS-CoV-2 spike protein is blocked by factor D inhibition
- Year:
- 2020
- Journal Name:
- Blood
SARS-CoV-2 spike protein reduces burst activities in neurons measured by micro-electrode arrays
- Year:
- 2023
- Journal Name:
- Annals of Medicine and Surgery
New Monoclonal Antibodies Specific for Different Epitopes of the Spike Protein of SARS-CoV-2 and Its Major Variants: Additional Tools for a More Specific COVID-19 Diagnosis
- Year:
- 2023
- Journal Name:
- Biomedicines
Piezoelectric point-of-care biosensor for the detection of SARS-COV-2 (COVID-19) antibodies
- Year:
- 2022
- Journal Name:
- Sensing and Bio-Sensing Research
SARS-CoV-2 spike 1 protein controls natural killer cell activation via the HLA-E/NKG2A pathway
- Year:
- 2020
- Journal Name:
- Cells
Oral Immunization with rVSV Bivalent Vaccine Elicits Protective Immune Responses, Including ADCC, against Both SARS-CoV-2 and Influenza A Viruses
- Year:
- 2023
- Journal Name:
- Vaccines



